Ploczeková C
Ustav preventívnej a klinickej medicíny, Bratislava.
Cesk Epidemiol Mikrobiol Imunol. 1992 Apr;41(1):8-14.
The author describes the preparation of polyclonal rabbit antibodies against gentamicin. As immunogens gentamicin conjugates with bovine serum albumin were used, or else with thyroglobulin and haemocyanin. The immunization pattern involved combined administration of immunogens by the intravenous route, or by the intramuscular or intradermal route, using liposomes and complete Freund adjuvant. The highest antibody titres against gentamicin found by the ELISA method were obtained when procedures were used where the carrier was serum albumin and thyroglobulin and as adjuvant complete Freund adjuvant was used.
作者描述了抗庆大霉素兔多克隆抗体的制备方法。使用庆大霉素与牛血清白蛋白的缀合物作为免疫原，也可使用与甲状腺球蛋白和血蓝蛋白的缀合物。免疫模式包括通过静脉途径、肌肉途径或皮内途径联合给予免疫原，同时使用脂质体和弗氏完全佐剂。当使用血清白蛋白和甲状腺球蛋白作为载体且弗氏完全佐剂作为佐剂的方法时，通过ELISA法获得的抗庆大霉素抗体效价最高。
