通过使用针对16S rRNA基因的定量PCR测定法快速估算粪便拟杆菌的数量。
Rapid estimation of numbers of fecal Bacteroidetes by use of a quantitative PCR assay for 16S rRNA genes.
作者信息
Dick Linda K, Field Katharine G
机构信息
Department of Microbiology, Oregon State University, Corvallis, OR 97331, USA.
出版信息
Appl Environ Microbiol. 2004 Sep;70(9):5695-7. doi: 10.1128/AEM.70.9.5695-5697.2004.
Abstract
Assessment of health risk associated with fecal pollution requires a reliable fecal indicator and a rapid quantification method. We report the development of a Taq nuclease assay for enumeration of 16S rRNA genes of Bacteroidetes. Sensitivity and correlation with standard fecal indicators provide experimental evidence for application of the assay in monitoring fecal pollution.
摘要
评估与粪便污染相关的健康风险需要一种可靠的粪便指示物和一种快速定量方法。我们报告了一种用于定量拟杆菌门16S rRNA基因的Taq核酸酶检测方法的开发。该检测方法的灵敏度及其与标准粪便指示物的相关性为其在监测粪便污染中的应用提供了实验证据。
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