Amills Marcel, Capote Juan, Tomàs Anna, Kelly Lucía, Obexer-Ruff Gabriela, Angiolillo Antonella, Sanchez Armand
Department de Ciència Animal i dels Aliments, Universitat Autbnoma de Barcelona, Bellaterra 08193, Spain.
J Dairy Res. 2004 Aug;71(3):257-62. doi: 10.1017/s0022029904000342.
We partially sequenced the mitochondrial D-loop region in 47 individuals from eleven Spanish and foreign goat breeds. Phylogenetic analysis of these sequences allowed us to identify a particular D-loop haplotype shared by individuals from the Palmera, Majorera and Tinerfeña Canarian breeds. Genotyping of 281 goats from 17 different breeds by PCR-Hpall RFLP evidenced that the geographical distribution of this haplotype is restricted to the Canary Islands. This ancestral mitochondrial haplotype might originate in the domestic goat herds brought by the native Canarian inhabitants approximately 3000 years ago. Although we observed other miscellaneous D-loop haplotypes in the Palmera, Majorera and Tinerfeña breeds, any of them allowed us to group individuals from these three populations in a single cluster, a feature that suggests that these haplotypes might have diverse origins. The remarkable degree of phylogeographic structure of the Canary goat breeds with regard to other Spanish and foreign populations might be attributed to the isolation of these breeds in the Canary Islands for approximately 2500 years, without exposure to the migratory movements and commercial trading events that probably affected the genesis of most domestic goat breeds worldwide. The Canarian D-loop haplotype can be efficiently genotyped by using DNA isolated from milk and cheese samples, which paves the way for the future establishment of a Canary breed identity test for these dairy products.
我们对来自11个西班牙及外国山羊品种的47只个体的线粒体D环区域进行了部分测序。对这些序列进行系统发育分析，使我们能够识别出帕尔梅拉、马约雷拉和廷内费纳加那利品种个体共有的一种特定D环单倍型。通过PCR-Hpall RFLP对来自17个不同品种的281只山羊进行基因分型，结果表明这种单倍型的地理分布仅限于加那利群岛。这种古老的线粒体单倍型可能起源于大约3000年前加那利群岛原住民带来的家养山羊群。尽管我们在帕尔梅拉、马约雷拉和廷内费纳品种中观察到了其他杂合的D环单倍型，但没有一种能让我们将这三个种群的个体归为一个聚类，这一特征表明这些单倍型可能有不同的起源。加那利山羊品种相对于其他西班牙及外国种群而言，具有显著的系统地理结构，这可能归因于这些品种在加那利群岛隔离了约2500年，未受到可能影响全球大多数家养山羊品种起源的迁徙活动和商业贸易事件的影响。利用从牛奶和奶酪样品中分离的DNA，可以有效地对加那利D环单倍型进行基因分型，这为未来建立针对这些乳制品的加那利品种身份检测铺平了道路。
