Yeang Hoong-Yeet, Arif Siti Arija M, Raulf-Heimsoth Monika, Loke Yin-Ho, Sander Ingrid, Sulong Siti Hawa, Lau Chee-Heng, Hamilton Robert G
Rubber Research Institute of Malaysia, 50908 Kuala Lumpur, Malaysia.
J Allergy Clin Immunol. 2004 Sep;114(3):593-8. doi: 10.1016/j.jaci.2004.05.039.
Sensitization to natural rubber latex has been linked to proteins from medical latex gloves. Various assays to estimate the amount of residual allergenic proteins extractable from latex gloves to assess their potential exposure hazard have inherent weaknesses.
This investigation was aimed at developing 2-site immunoenzymetric assays and identifying appropriate protein markers to assess the allergenic potential of latex gloves.
The presence of 6 latex allergens--Hev b 1, 2, 3, 5, 6, and 13--was measured in a cross-section of commercial latex medical gloves by using monoclonal and polyclonal antibody-based 2-site immunoenzymetric assays. The overall allergenic potential of these gloves was assessed by IgE-inhibition assay. Stepwise multiple regression analyses were performed to identify marker allergens that best explained the variation in latex glove allergenicity.
All 6 latex allergens were detected in at least some of the glove samples. Hev b 5 and Hev b 13 were identified as the marker allergens that combined best to explain the variation in the glove allergenicity. The significant multiple correlation (R=0.855) between these 2 markers and glove allergenic potency forms the basis of an assay to gauge latex glove allergenicity.
The overall allergenic potential of latex gloves can be estimated by using Hev b 5 and Hev b 13 as indicator allergens. The correlation between glove allergenicity and the level of these allergens was maintained for low-protein gloves (<200 microg/g). This estimation of glove allergenicity was superior to that obtained by using total protein readings.
对天然橡胶乳胶的致敏作用与医用乳胶手套中的蛋白质有关。各种用于估计可从乳胶手套中提取的残留致敏蛋白量以评估其潜在暴露风险的检测方法都存在固有缺陷。
本研究旨在开发双位点免疫酶测定法并确定合适的蛋白质标志物，以评估乳胶手套的致敏潜力。
通过基于单克隆和多克隆抗体的双位点免疫酶测定法，对一系列商用乳胶医用手套进行检测，测定其中6种乳胶过敏原——Hev b 1、2、3、5、6和13的存在情况。通过IgE抑制试验评估这些手套的总体致敏潜力。进行逐步多元回归分析，以确定最能解释乳胶手套致敏性差异的标志物过敏原。
在至少部分手套样品中检测到了所有6种乳胶过敏原。Hev b 5和Hev b 13被确定为最能共同解释手套致敏性差异的标志物过敏原。这两种标志物与手套致敏效力之间的显著多重相关性（R = 0.855）构成了一种评估乳胶手套致敏性检测方法的基础。
可以使用Hev b 5和Hev b 13作为指示性过敏原，来估计乳胶手套的总体致敏潜力。对于低蛋白手套（<200微克/克），手套致敏性与这些过敏原水平之间的相关性依然存在。这种对手套致敏性的估计优于通过总蛋白读数获得的估计。
