沃尔斯比方形嗜盐古菌的培养

Cultivation of Walsby's square haloarchaeon.

作者信息

Burns David G, Camakaris Helen M, Janssen Peter H, Dyall-Smith Mike L

机构信息

Department of Microbiology and Immunology, University of Melbourne, Vic. 3010, Australia.

出版信息

FEMS Microbiol Lett. 2004 Sep 15;238(2):469-73. doi: 10.1016/j.femsle.2004.08.016.

DOI:10.1016/j.femsle.2004.08.016

PMID:15358434

Abstract

The square haloarchaea of Walsby (SHOW group) dominate hypersaline microbial communities but have not been cultured since their discovery 25 years ago. We show that natural water dilution cultures can be used to isolate members of this group and, once in pure culture, they can be grown in standard halobacterial media. Cells display a square morphology and contain gas vesicles and poly-beta-hydroxybutyrate (PHB) granules. The 16S rRNA gene sequence was >99% identical to other SHOW group sequences. They prefer high salinities (23-30%), and can grow with a doubling time of 1-2 days in rich media. The ability to culture SHOW group organisms makes it possible to study, in a comprehensive way, the microbial ecology of salt lakes.

摘要

瓦尔兹比的方形嗜盐古菌（SHOW组）在高盐度微生物群落中占主导地位，但自25年前被发现以来一直未被培养出来。我们表明，天然水稀释培养可用于分离该组的成员，一旦处于纯培养状态，它们就能在标准嗜盐细菌培养基中生长。细胞呈现方形形态，含有气体泡囊和聚-β-羟基丁酸酯（PHB）颗粒。16S rRNA基因序列与其他SHOW组序列的相似度>99%。它们偏好高盐度（23 - 30%），在丰富培养基中生长时倍增时间为1 - 2天。培养SHOW组生物的能力使得全面研究盐湖的微生物生态学成为可能。