School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Department of Microbiology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran 14178-64411, Iran.
Int J Syst Evol Microbiol. 2024 Mar;74(3). doi: 10.1099/ijsem.0.006290.
Halophilic archaea of the class are the most salt-requiring prokaryotes within the domain . In 1997, minimal standards for the description of new taxa in the order were proposed. From then on, the taxonomy of the class provides an excellent example of how changing concepts on prokaryote taxonomy and the development of new methods were implemented. The last decades have witnessed a rapid expansion of the number of described taxa within the class coinciding with the era of genome sequencing development. The current members of the International Committee on Systematics of Prokaryotes Subcommittee on the Taxonomy of propose these revisions to the recommended minimal standards and encourage the use of advanced technologies in the taxonomic description of members of the . Most previously required and some recommended minimal standards for the description of new taxa in the class were retained in the present revision, but changes have been proposed in line with the new methodologies. In addition to the 16S rRNA gene, the gene is an important molecular marker for the identification of members of the . Phylogenomic analysis based on concatenated conserved, single-copy marker genes is required to infer the taxonomic status of new taxa. The overall genome relatedness indexes have proven to be determinative in the classification of the taxa within the class . Average nucleotide identity, digital DNA-DNA hybridization, and average amino acid identity values should be calculated for rigorous comparison among close relatives.
嗜盐古菌是域内最需要盐的原核生物。1997 年，人们为该目下的新分类单元提出了最低标准描述建议。从那时起，该纲的分类为原核生物分类学概念的变化以及新方法的发展提供了一个很好的范例。在过去的几十年中，随着基因组测序技术的发展，该纲内被描述的分类单元数量迅速增加。国际系统学委员会下属的 分类小组委员会的现任成员提议对建议的最低标准进行修订，并鼓励在该纲成员的分类描述中使用先进技术。大多数以前要求的和一些推荐的该纲新分类单元的最低标准在本次修订中得以保留，但也根据新方法提出了一些修改。除了 16S rRNA 基因外，基因也是鉴定该纲成员的重要分子标记。基于串联的保守、单拷贝标记基因的系统发育分析对于推断新分类单元的分类地位是必需的。全基因组相关指数已被证明在该纲内分类单元的分类中具有决定性。平均核苷酸同一性、数字 DNA-DNA 杂交和平均氨基酸同一性值应计算以进行近亲之间的严格比较。
