Sauren Y M, Mieremet R H, Groot C G, Scherft J P
Laboratory of Cell Biology and Histology, University of Leiden, The Netherlands.
Anat Rec. 1992 Jan;232(1):36-44. doi: 10.1002/ar.1092320105.
The presence of proteoglycans (PGs) was studied in compact lamellar rat and human bone at the electron microscopic level. With the cationic dye cuprolinic blue (CB1), PGs could be demonstrated in the mineralized bone matrix. The amounts of PGs appeared to be equal in the different lamellae and osteons. More CBl-positive material was found in the outermost lamella of the cortex, in the perilacunar matrix around the osteocyte lacunae, and around the canaliculi. Enzyme digestion with chondroitinase ABC demonstrated that the CBl-positive rods consisted of PGs. These observations amplify biochemical studies in which PGs have been isolated from the mineralized bone matrix. The presence of CBl-positive rods in the mineralized matrix suggest that PGs do not have to be removed completely to make the matrix calcifiable.
在电子显微镜水平上研究了蛋白聚糖（PGs）在大鼠和人类致密板层骨中的存在情况。用阳离子染料铜啉蓝（CB1）可在矿化骨基质中显示出PGs。不同板层和骨单位中的PGs含量似乎相等。在皮质的最外层板层、骨细胞陷窝周围的陷窝周围基质以及小管周围发现了更多的CB1阳性物质。用软骨素酶ABC进行酶消化表明，CB1阳性杆状物由PGs组成。这些观察结果扩展了从矿化骨基质中分离出PGs的生化研究。矿化基质中CB1阳性杆状物的存在表明，PGs不必完全去除就能使基质钙化。
