通过聚合酶链反应（PCR）和测序对人腺病毒进行全面检测和血清分型
Comprehensive detection and serotyping of human adenoviruses by PCR and sequencing.
作者信息
Sarantis Helen, Johnson Grant, Brown Martha, Petric Martin, Tellier Raymond
机构信息
Division of Microbiology, The Hospital for Sick Children, 555 University Ave., Toronto, Ontario M5G 1X8, Canada.
出版信息
J Clin Microbiol. 2004 Sep;42(9):3963-9. doi: 10.1128/JCM.42.9.3963-3969.2004.
Abstract
Human adenoviruses are common pathogens associated with many diseases, including respiratory, gastrointestinal, and ocular infections. Because they are now being increasingly recognized as agents of life-threatening disseminated infection in immunocompromised patients, robust and sensitive laboratory detection methods are needed for their rapid diagnosis. We describe here a PCR assay using a single primer pair, targeting a region of the hexon gene containing hypervariable region 7, that can detect all known human adenovirus serotypes and allows for serotype determination through the analysis of the nucleotide sequence. This comprehensive assay has proven effective for diagnosing adenoviruses at the serotype level in a broad range of patient specimens, including conjunctival, nasopharyngeal, stool, blood, and urine specimens.
摘要
人腺病毒是与多种疾病相关的常见病原体，包括呼吸道、胃肠道和眼部感染。由于它们现在越来越被认为是免疫功能低下患者危及生命的播散性感染的病原体，因此需要强大而灵敏的实验室检测方法来进行快速诊断。我们在此描述一种聚合酶链反应（PCR）检测方法，该方法使用一对引物，靶向包含高变区7的六邻体基因区域，能够检测所有已知的人腺病毒血清型，并通过核苷酸序列分析确定血清型。这种全面的检测方法已被证明在诊断多种患者标本中的腺病毒血清型方面是有效的，这些标本包括结膜、鼻咽、粪便、血液和尿液标本。
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