NAD 依赖性乳酸脱氢酶在植物乳杆菌稳定期早期有氧利用乳酸中的主要作用
Major role of NAD-dependent lactate dehydrogenases in aerobic lactate utilization in Lactobacillus plantarum during early stationary phase.
作者信息
Goffin Philippe, Lorquet Frédérique, Kleerebezem Michiel, Hols Pascal
机构信息
Unité de Génétique, Institut des Sciences de la Vie, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.
出版信息
J Bacteriol. 2004 Oct;186(19):6661-6. doi: 10.1128/JB.186.19.6661-6666.2004.
Abstract
NAD-independent lactate dehydrogenases are commonly thought to be responsible for lactate utilization during the stationary phase of aerobic growth in Lactobacillus plantarum. To substantiate this view, we constructed single and double knockout mutants for the corresponding genes, loxD and loxL. Lactate-to-acetate conversion was not impaired in these strains, while it was completely blocked in mutants deficient in NAD-dependent lactate dehydrogenase activities, encoded by the ldhD and ldhL genes. We conclude that NAD-dependent but not NAD-independent lactate dehydrogenases are involved in this process.
摘要
不依赖NAD的乳酸脱氢酶通常被认为在植物乳杆菌有氧生长的稳定期负责乳酸的利用。为了证实这一观点，我们构建了相应基因loxD和loxL的单敲除和双敲除突变体。在这些菌株中，乳酸到乙酸的转化没有受到损害，而在由ldhD和ldhL基因编码的缺乏依赖NAD的乳酸脱氢酶活性的突变体中，这种转化被完全阻断。我们得出结论，参与这一过程的是依赖NAD的乳酸脱氢酶，而不是不依赖NAD的乳酸脱氢酶。
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2
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3
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4
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Biochim Biophys Acta. 1963 Sep 3;77:7-9. doi: 10.1016/0006-3002(63)90464-5.
5
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6
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J Biol Chem. 1960 Mar;235:810-8.
7
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8
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10
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