Van Driessche Ellen, Houf Kurt, Vangroenweghe Frédéric, Nollet Nathalie, De Zutter Lieven, Vandamme Peter, Van Hoof Jan
Department of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
Res Microbiol. 2004 Oct;155(8):662-6. doi: 10.1016/j.resmic.2004.04.011.
In this study, Arcobacter species were isolated from clinically healthy porkers and sows on four unrelated pig farms, using a quantitative isolation protocol. Isolates were identified by m-PCR, and fingerprints were distinguished by modified ERIC-PCR. The prevalence of Arcobacter in pigs ranged from 16 to 85%. Arcobacter excretion ranged from 0 to 10(4) CFUg(-1) feces. Arcobacter butzleri was the most frequently occurring species, but simultaneous shedding of two or three species occurred. Large heterogeneity among the Arcobacter species was detected in pigs and on the farms.
在本研究中，采用定量分离方案，从四个无关联猪场的临床健康仔猪和母猪中分离出弓形杆菌属菌株。通过多重聚合酶链反应（m-PCR）鉴定分离菌株，并通过改良的肠杆菌基因间重复共有序列聚合酶链反应（ERIC-PCR）区分指纹图谱。猪中弓形杆菌的流行率为16%至85%。弓形杆菌排泄量为0至10⁴CFU/g粪便。布氏弓形杆菌是最常出现的菌种，但也会同时排出两种或三种菌种。在猪和猪场中检测到弓形杆菌属菌种之间存在很大的异质性。
