Chinivasagam H N, Corney B G, Wright L L, Diallo I S, Blackall P J
Department of Primary Industries and Fisheries, Animal Research Institute, Queensland, Australia.
J Appl Microbiol. 2007 Aug;103(2):418-26. doi: 10.1111/j.1365-2672.2007.03275.x.
To investigate the occurrence and levels of Arcobacter spp. in pig effluent ponds and effluent-treated soil.
A Most Probable Number (MPN) method was developed to assess the levels of Arcobacter spp. in seven pig effluent ponds and six effluent-treated soils, immediately after effluent irrigation. Arcobacter spp. levels in the effluent ponds varied from 6.5 x 10(5) to 1.1 x 10(8) MPN 100 ml(-1) and in freshly irrigated soils from 9.5 x 10(2) to 2.8 x 10(4) MPN g(-1) in all piggery environments tested. Eighty-three Arcobacter isolates were subjected to an abbreviated phenotypic test scheme and examined using a multiplex polymerase chain reaction (PCR). The PCR identified 35% of these isolates as Arcobacter butzleri, 49% as Arcobacter cryaerophilus while 16% gave no band. All 13 nonreactive isolates were subjected to partial 16S rDNA sequencing and showed a high similarity (>99%) to Arcobacter cibarius.
A. butzleri, A. cryaerophilus and A. cibarius were isolated from both piggery effluent and effluent-irrigated soil, at levels suggestive of good survival in the effluent pond.
This is the first study to provide quantitative information on Arcobacter spp. levels in piggery effluent and to associate A. cibarius with pigs and piggery effluent environments.
调查猪废水池塘及废水处理后土壤中嗜冷弧菌属的存在情况及含量水平。
开发了一种最大可能数（MPN）法，用于评估七个猪废水池塘和六个废水处理后土壤中嗜冷弧菌属的含量水平，在废水灌溉后立即进行检测。在所测试的所有养猪场环境中，废水池塘中嗜冷弧菌属的含量水平在6.5×10⁵至1.1×10⁸ MPN 100 ml⁻¹之间，新灌溉土壤中的含量水平在9.5×10²至2.8×10⁴ MPN g⁻¹之间。对83株嗜冷弧菌分离株进行了简化的表型测试方案，并使用多重聚合酶链反应（PCR）进行检测。PCR鉴定出35%的分离株为布氏嗜冷弧菌，49%为嗜低温嗜冷弧菌，而16%未出现条带。对所有13株无反应的分离株进行了16S rDNA部分测序，结果显示与西巴嗜冷弧菌具有高度相似性（>99%）。
从养猪场废水和废水灌溉土壤中均分离出布氏嗜冷弧菌、嗜低温嗜冷弧菌和西巴嗜冷弧菌，其含量水平表明它们在废水池塘中具有良好的生存能力。
这是第一项提供养猪场废水中嗜冷弧菌属含量水平定量信息，并将西巴嗜冷弧菌与猪及养猪场废水环境相关联的研究。
