华支睾吸虫韩国和中国分离株的核DNA和线粒体DNA变异
Variation of nuclear and mitochondrial DNAs in Korean and Chinese isolates of Clonorchis sinensis.
作者信息
Lee Soo-Ung, Huh Sun
机构信息
Department of Parasitology, College of Medicine, Hallym University, Chuncheon 200-702, Repubic of Korea.
出版信息
Korean J Parasitol. 2004 Sep;42(3):145-8. doi: 10.3347/kjp.2004.42.3.145.
Abstract
We compared the DNA sequence difference of isolates of Clonorchis sinensis from one Korean (Kimhae) and two Chinese areas (Guangxi and Shenyang). The sequences of nuclear rDNA (18S, internal transcribed spacer 1 and 2: ITS1 and ITS2) and mitochondrial DNA (cytochrome c oxidase subunit 1: cox1) were compared. A very few intraspecific nucleotide substitution of the 18S, ITS1, ITS2 and cox1 was found among three isolates of C. sinensis and a few nucleotide insertion and deletion of ITS1 were detected. The 18S, ITS1, ITS2 and cox1 sequences were highly conserved among three isolates. These findings indicated that the Korean and two Chinese isolates are similar at the DNA sequence level.
摘要
我们比较了来自韩国（金海）以及中国两个地区（广西和沈阳）的华支睾吸虫分离株的DNA序列差异。对核糖体DNA（18S、内部转录间隔区1和2：ITS1和ITS2）和线粒体DNA（细胞色素c氧化酶亚基1：cox1）的序列进行了比较。在华支睾吸虫的三个分离株中，18S、ITS1、ITS2和cox1的种内核苷酸替换极少，并且检测到ITS1有少量核苷酸插入和缺失。18S、ITS1、ITS2和cox1序列在三个分离株中高度保守。这些发现表明，韩国和中国的两个分离株在DNA序列水平上相似。
相似文献
1
Variation of nuclear and mitochondrial DNAs in Korean and Chinese isolates of Clonorchis sinensis.
Korean J Parasitol. 2004 Sep;42(3):145-8. doi: 10.3347/kjp.2004.42.3.145.
2
Geographical variation of the liver fluke, Clonorchis sinensis, from Korea and China based on the karyotypes, zymodeme and DNA sequences.
Southeast Asian J Trop Med Public Health. 2001;32 Suppl 2:12-6.
3
Genetic comparison of liver flukes, Clonorchis sinensis and Opisthorchis viverrini, based on rDNA and mtDNA gene sequences.
Parasitol Res. 2007 Jan;100(2):351-7. doi: 10.1007/s00436-006-0269-x. Epub 2006 Aug 11.
4
Genetic variation among Clonorchis sinensis isolates from different hosts and geographical locations revealed by sequence analysis of mitochondrial and ribosomal DNA regions.
Mitochondrial DNA. 2013 Oct;24(5):559-64. doi: 10.3109/19401736.2013.770490. Epub 2013 Mar 6.
5
Molecular characterisation of Sarcocystis bovifelis, Sarcocystis bovini n. sp., Sarcocystis hirsuta and Sarcocystis cruzi from cattle (Bos taurus) and Sarcocystis sinensis from water buffaloes (Bubalus bubalis).
Parasitol Res. 2016 Apr;115(4):1473-92. doi: 10.1007/s00436-015-4881-5. Epub 2015 Dec 17.
6
Genetic diversity of nuclear ITS1-5.8S-ITS2 rDNA sequence in Clonorchis sinensis Cobbold, 1875 (Trematoda: Opisthorchidae) from the Russian Far East.
Parasitol Int. 2012 Dec;61(4):664-74. doi: 10.1016/j.parint.2012.07.005. Epub 2012 Jul 21.
7
Comparative analyses of complete ribosomal DNA sequences of Clonorchis sinensis and Metorchis orientalis: IGS sequences may provide a novel genetic marker for intraspecific variation.
Infect Genet Evol. 2020 Mar;78:104125. doi: 10.1016/j.meegid.2019.104125. Epub 2019 Nov 23.
8
Molecular characterization and phylogeny of whipworm nematodes inferred from DNA sequences of cox1 mtDNA and 18S rDNA.
Parasitol Res. 2013 Nov;112(11):3933-49. doi: 10.1007/s00436-013-3584-z. Epub 2013 Sep 10.
9
Comparative study on DNA sequences of ribosomal DNA and cytochrome c oxidase subunit 1 of mitochondrial DNA among five species of gnathostomes.
J Helminthol. 2006 Mar;80(1):7-13. doi: 10.1079/joh2005315.
10
Fasciolopsis buski (Digenea: Fasciolidae) from China and India may represent distinct taxa based on mitochondrial and nuclear ribosomal DNA sequences.
Parasit Vectors. 2017 Feb 22;10(1):101. doi: 10.1186/s13071-017-2039-2.
引用本文的文献
1
Genetic Variation and Population Structure of : An In Silico Analysis.
Pathogens. 2024 Nov 13;13(11):991. doi: 10.3390/pathogens13110991.
2
Liver Flukes: Clonorchis and Opisthorchis.
Adv Exp Med Biol. 2024;1454:239-284. doi: 10.1007/978-3-031-60121-7_7.
3
Black spot diseases in seven commercial fish species from the English Channel and the North Sea: infestation levels, identification and population genetics of Cryptocotyle spp.
Parasite. 2023;30:28. doi: 10.1051/parasite/2023028. Epub 2023 Jul 6.
4
Ancient DNA of Metagonimus yokogawai Recovered from Joseon Period Human Remains Newly Discovered at Goryeong County in South Korea.
Acta Parasitol. 2022 Mar;67(1):539-545. doi: 10.1007/s11686-021-00487-0. Epub 2021 Nov 3.
5
Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod (Gadus morhua) from Danish seas and whiting (Merlangius merlangus) from the English Channel.
Parasitol Res. 2021 Oct;120(10):3417-3427. doi: 10.1007/s00436-021-07278-6. Epub 2021 Aug 27.
6
Morphological and Molecular Characterization of Paragonimus skrjabini Complex from Yunnan, China: A Brief Report.
Acta Parasitol. 2022 Mar;67(1):316-321. doi: 10.1007/s11686-021-00461-w. Epub 2021 Aug 21.
7
Marked mitochondrial genetic variation in individuals and populations of the carcinogenic liver fluke Clonorchis sinensis.
PLoS Negl Trop Dis. 2020 Aug 19;14(8):e0008480. doi: 10.1371/journal.pntd.0008480. eCollection 2020 Aug.
8
Cytochrome C Oxidase Subunit 1, Internal Transcribed Spacer 1, Nicotinamide Adenine Dinucleotide Hydrogen Dehydrogenase Subunits 2 and 5 of Ancient DNA Retrieved from Joseon Dynasty Mummy Specimens.
J Korean Med Sci. 2019 May 27;34(20):e149. doi: 10.3346/jkms.2019.34.e149.
9
Molecular Identification and Phylogenetic Analysis of Nuclear rDNA Sequences of Isolates From Human Fecal Samples in Heilongjiang Province, China.
Front Microbiol. 2019 Jan 28;10:26. doi: 10.3389/fmicb.2019.00026. eCollection 2019.
10
Application of a loop-mediated isothermal amplification (LAMP) assay targeting cox1 gene for the detection of Clonorchis sinensis in human fecal samples.
PLoS Negl Trop Dis. 2017 Oct 9;11(10):e0005995. doi: 10.1371/journal.pntd.0005995. eCollection 2017 Oct.
本文引用的文献
1
The current pathobiology and chemotherapy of clonorchiasis.
Kisaengchunghak Chapchi. 1986 Apr;24 Suppl:1-141. doi: 10.3347/kjp.1986.24.suppl.1.
2
Nucleotide sequence of mitochondrial CO I and ribosomal ITS II genes of Opisthorchis viverrini in northeast Thailand.
Southeast Asian J Trop Med Public Health. 2001;32 Suppl 2:17-22.
3
Geographical variation of the liver fluke, Clonorchis sinensis, from Korea and China based on the karyotypes, zymodeme and DNA sequences.
Southeast Asian J Trop Med Public Health. 2001;32 Suppl 2:12-6.
4
Relative merits of nuclear ribosomal internal transcribed spacers and mitochondrial CO1 and ND1 genes for distinguishing among Echinostoma species (Trematoda).
Parasitology. 1998 Mar;116 ( Pt 3):289-97. doi: 10.1017/s0031182097002217.
5
Molecular phylogeny of Schistosoma species supports traditional groupings within the genus.
J Parasitol. 1996 Apr;82(2):292-8.
6
Nuclear and mitochondrial genetic markers highly conserved between Chinese and Philippine Schistosoma japonicum.
Acta Trop. 1993 Dec;55(4):217-29. doi: 10.1016/0001-706x(93)90079-q.
7
A molecular phylogeny of the human schistosomes.
Mol Phylogenet Evol. 1995 Jun;4(2):103-9. doi: 10.1006/mpev.1995.1011.
8
CLUSTAL V: improved software for multiple sequence alignment.
Comput Appl Biosci. 1992 Apr;8(2):189-91. doi: 10.1093/bioinformatics/8.2.189.
9
Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing.
Mol Biochem Parasitol. 1992 Sep;54(2):165-73. doi: 10.1016/0166-6851(92)90109-w.
10
DNA sequencing with chain-terminating inhibitors.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7. doi: 10.1073/pnas.74.12.5463.
Zotero 插件