Department of Mortuary Science, College of Bio-Convergence, Eulji University, 553, Sanseongdae-ro, Sujeong-gu, Seongnam-si, Gyeonggi-do, 13135, South Korea.
Institute of Korean Archaeology and Ancient History, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, South Korea.
Acta Parasitol. 2022 Mar;67(1):539-545. doi: 10.1007/s11686-021-00487-0. Epub 2021 Nov 3.
Metagonimiasis, commonly seen in East Asian countries, is a parasitic disorder caused by definitive hosts' ingestion of undercooked freshwater fishes. Recently, genetic analysis has proved 28S rRNA and cytochrome c oxidase subunit I (COI) mtDNA gene to be a successful marker differentiating species of the genus Metagonimus. In the present study, using specimens from the newly discovered Joseon Dynasty human remains of Goryeong, we obtained updated genetic data on genus Metagonimus, which was also prevalent during the Joseon period.
The ancient DNA (aDNA) was retrieved from the coprolite sample of the seventeenth century, half-mummified individual discovered at Goryeong Country, South Korea. Cloning and sequencing were performed on PCR-amplified amplicons for M. yokogawai 28S rRNA and COI mtDNA gene. The consensus sequences were used for species identification and phylogenetic analysis using NCBI/BLAST and MEGA X software.
Based on the COI mtDNA gene region, the Goryeong sequence was confirmed as belonging to M. yokogawai, as it was shown to form a separate cluster with other M. yokogawai taxa that are distinct also from M. takahashii and M. miyatai.
In a series of our genetic analyses on genus Metagonimus using samples retrieved from Joseon-period cases, aDNA sequences of genus Metagonimus revealed in South Korea thus far are those of M. yokogawai, but not of M. miyatai or M. takahashii yet.
东亚洲国家常见的并殖吸虫病是一种寄生虫病，由终宿主食用未煮熟的淡水鱼引起。最近，遗传分析证明 28S rRNA 和细胞色素 c 氧化酶亚基 I (COI) mtDNA 基因是区分并殖属物种的成功标记。本研究利用新发现的高丽时代遗骸中的标本，获得了在朝鲜高丽时代也流行的并殖属的最新遗传数据。
从古高丽国发现的半木乃伊化的 17 世纪粪便样本中提取古 DNA (aDNA)。对 PCR 扩增的 M. yokogawai 28S rRNA 和 COI mtDNA 基因进行克隆和测序。使用 NCBI/BLAST 和 MEGA X 软件对共识序列进行物种鉴定和系统发育分析。
基于 COI mtDNA 基因区，高丽序列被确认为 M. yokogawai，因为它与其他 M. yokogawai 分类群形成了一个单独的聚类，也与 M. takahashii 和 M. miyatai 明显不同。
在使用从高丽时期病例中提取的样本对并殖属进行的一系列遗传分析中，迄今为止在韩国发现的并殖属的 aDNA 序列是 M. yokogawai 的，但不是 M. miyatai 或 M. takahashii 的。
