五种颌口类动物核糖体DNA和线粒体细胞色素c氧化酶亚基1的DNA序列比较研究
Comparative study on DNA sequences of ribosomal DNA and cytochrome c oxidase subunit 1 of mitochondrial DNA among five species of gnathostomes.
作者信息
Ando K, Tsunemori M, Akahane H, Tesana S, Hasegawa H, Chinzei Y
机构信息
Department of Medical Zoology, School of Medicine, Mie University, Tsu 514-8507, Japan.
出版信息
J Helminthol. 2006 Mar;80(1):7-13. doi: 10.1079/joh2005315.
The nucleotide sequences of partial 18S, complete internal transcribed spacer region 1 (ITS1), complete 5.8S, complete ITS2 and partial 28S of ribosomal DNA (rDNA) and cytochrome c oxidase subunit 1 of mitochondrial DNA (MCOI) from five species of gnathostomes (G. spinigerum, G. doloresi, G. nipponicum, G. hispidum and G. binucleatum with the former four species being distributed in Japan and Asia) that cause human gnathostomiasis were compared by direct polymerase chain reaction cycle-sequencing. The nucleotide sequences of each region of the18S (613 bp), 5.8S (158 bp) and 28S (598 bp) rDNA from the five species were almost identical. The ITS1 region was different in length for the five species. The nucleotide sequences of each region of ITS2 and partial MCO1 regions were different among the five species. Therefore, these two regions can be used as genetic markers for identification of worms.
通过直接聚合酶链反应循环测序，比较了五种引起人类颚口线虫病的颚口线虫（棘颚口线虫、多氏颚口线虫、日本颚口线虫、刚刺颚口线虫和双核颚口线虫，前四种分布于日本和亚洲）核糖体DNA（rDNA）的部分18S、完整的内部转录间隔区1（ITS1）、完整的5.8S、完整的ITS2和部分28S以及线粒体DNA的细胞色素c氧化酶亚基1（MCOI）的核苷酸序列。这五个物种的18S（613 bp）、5.8S（158 bp）和28S（598 bp）rDNA各区域的核苷酸序列几乎相同。五个物种的ITS1区域长度不同。五个物种的ITS2各区域和部分MCO1区域的核苷酸序列不同。因此，这两个区域可作为鉴定蠕虫的遗传标记。
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