Emerging Bacterial Pathogens Unit, San Raffaele Scientific Institute, Milan, Italy.
Department of Clinical Bacteriology, Parasitology, Zoonoses and Geographical Medicine, University Hospital of Heraklion, Heraklion, Greece.
Int J Syst Evol Microbiol. 2013 Feb;63(Pt 2):401-411. doi: 10.1099/ijs.0.038737-0. Epub 2012 Mar 23.
A thorough phenotypic and genotypic analysis of 150 strains belonging to the Mycobacterium terrae complex resulted in the identification of a number of previously unreported sequevars (sqvs) within the species known to belong to the complex. For the species Mycobacterium arupense, three sqvs were detected in the 16S rRNA gene, six sqvs in the hsp65 gene and 15 sqvs in the rpoB gene; in Mycobacterium senuense two sqvs were present in each of the three genetic regions; in Mycobacterium kumamotonense four, two and nine sqvs were found, respectively, and in M. terrae three, four and six sqvs were found, respectively. The inappropriate inclusion of Mycobacterium triviale within the M. terrae complex was confirmed. The limited utility of biochemical tests and of mycolic acid analyses for the differentiation of the members of M. terrae complex was also confirmed. The survey allowed the recognition of three previously undescribed species that were characterized by unique sequences in the 16S rRNA, hsp65 and rpoB genes. Mycobacterium engbaekii sp. nov. (proposed previously 40 years ago but never validly published) was characterized by pink photochromogenic pigmentation and rapid growth; phylogenetically it was related to Mycobacterium hiberniae. The type strain of this species, of which eight strains were investigated, is ATCC 27353(T) ( = DSM 45694(T)). A cluster of 24 strains was the basis for the description of Mycobacterium heraklionense sp. nov., which has an intermediate growth rate and is unpigmented; nitrate reductase activity is typically strong. Closely related to M. arupense with respect to the 16S rRNA gene, M. heraklionense sp. nov. could be clearly differentiated from the latter species in the other genetic regions investigated. The type strain is NCTC 13432(T) ( = LMG 24735(T) = CECT 7509(T)). Mycobacterium longobardum sp. nov., represented in the study by seven strains, was characterized by a unique phylogenetic location within the M. terrae complex, clearly divergent from any other species. The type strain is DSM 45394(T) ( = CCUG 58460(T)).
对属于分枝杆菌 terrae 复合体的 150 株菌株进行了全面的表型和基因型分析，结果在已知属于该复合体的种中鉴定出了一些以前未报道的序列变异（sqv）。对于种分枝杆菌 aurupense，在 16S rRNA 基因中检测到 3 个 sqv，在 hsp65 基因中检测到 6 个 sqv，在 rpoB 基因中检测到 15 个 sqv；在分枝杆菌 senense 中，在三个基因区域中分别存在 2 个 sqv；在分枝杆菌 kumamotonense 中，分别发现了 4、2 和 9 个 sqv，而在分枝杆菌 terrae 中，分别发现了 3、4 和 6 个 sqv。证实了将分枝杆菌 triviale 不恰当地归入分枝杆菌 terrae 复合体。还证实了生化试验和分枝菌酸分析在分枝杆菌 terrae 复合体成员的分化中的有限用途。该调查使人们能够识别出三种以前未描述的种，它们在 16S rRNA、hsp65 和 rpoB 基因中具有独特的序列。分枝杆菌 engbaekii sp. nov.（四十年前提出但从未正式发表）的特征是粉红色光致变色色素沉着和快速生长；系统发育上与分枝杆菌 hiberniae 有关。该种的模式株，共调查了 8 株，是 ATCC 27353（T）（=DSM 45694（T））。24 株菌的一个群落在描述分枝杆菌 heraklionense sp. nov.的基础上，该种生长速度中等，无色素沉着；硝酸盐还原酶活性通常很强。与 16S rRNA 基因有关的分枝杆菌 aurupense 密切相关，分枝杆菌 heraklionense sp. nov.在其他基因区域可以与后者明显区分开来。模式株是 NCTC 13432（T）（=LMG 24735（T）=CECT 7509（T））。分枝杆菌 longobardum sp. nov.在研究中由 7 株菌代表，其特征是在分枝杆菌 terrae 复合体中的独特进化位置，与任何其他种明显不同。模式株是 DSM 45394（T）（=CCUG 58460（T））。
