负责水稻中植保素、稻瘟菌素A、B以及水稻抗毒素A - F生物合成的二萜环化酶。

Diterpene cyclases responsible for the biosynthesis of phytoalexins, momilactones A, B, and oryzalexins A-F in rice.

作者信息

Otomo Kazuko, Kanno Yuri, Motegi Akihiro, Kenmoku Hiromichi, Yamane Hisakazu, Mitsuhashi Wataru, Oikawa Hideaki, Toshima Hiroaki, Itoh Hironori, Matsuoka Makoto, Sassa Takeshi, Toyomasu Tomonobu

机构信息

Department of Bioresource Engineering, Yamagata University, Tsuruoka, Japan.

出版信息

Biosci Biotechnol Biochem. 2004 Sep;68(9):2001-6. doi: 10.1271/bbb.68.2001.

DOI:10.1271/bbb.68.2001

PMID:15388982

Abstract

Rice (Oryza sativa L.) produces diterpene phytoalexins, such as momilactones, oryzalexins, and phytocassanes. Using rice genome information and in vitro assay with recombinant enzymes, we identified genes (OsKS4 and OsKS10) encoding the type-A diterpene cyclases 9beta-pimara-7,15-diene synthase and ent-sandaracopimaradiene synthase which are involved in the biosynthesis of momilactones A, B and oryzalexins A-F respectively. Transcript levels of these two genes increased remarkably after ultraviolet (UV) treatment, which is consistent with elevated production of phytoalexins by UV. These two genes might prove powerful tools for understanding plant defense mechanisms in rice.

摘要

水稻（Oryza sativa L.）能产生二萜植保素，如稻瘟菌素、水稻抗毒素和植二苯并呋喃。利用水稻基因组信息并通过重组酶进行体外测定，我们鉴定出了编码参与稻瘟菌素A、B以及水稻抗毒素A - F生物合成的A型二萜环化酶9β - 海松二烯 - 7,15 - 合酶和对映 - 山达海松二烯合酶的基因（OsKS4和OsKS10）。这两个基因的转录水平在紫外线（UV）处理后显著增加，这与UV诱导植保素产量升高相一致。这两个基因可能是理解水稻植物防御机制的有力工具。