Salem G J, Zernicke R F, Barnard R J
Department of Kinesiology, University of California, Los Angeles 90024-1568.
Am J Physiol. 1992 Feb;262(2 Pt 2):R318-21. doi: 10.1152/ajpregu.1992.262.2.R318.
High fat and sucrose (HFS) diets may induce glucose intolerance, alter calcium metabolism, and lead to deficits in bone mineralization, development, and mechanical properties. To determine the mechanical and structural consequences of a HFS diet on rapidly growing vertebrae, female Sprague-Dawley rats (8 wk) were assigned randomly (2:1) either to a control group (n = 20) fed a low-fat complex-carbohydrate diet or an experimental group (n = 10) fed a HFS diet for 10-12 wk. The sixth lumbar vertebral body (L6) was isolated from the pedicles, morphological measures were taken, and compression was tested at a fast strain rate, while immersed in a warmed (37 degrees C) isotonic physiological buffer solution. No significant difference in body mass existed between HFS and control groups; nevertheless, HFS L6 cross-sectional areas, lengths, and volumes were significantly smaller than controls. The HFS L6 also had significantly lower mechanical properties, including initial maximum load, energy at initial maximum load, and strain energy density at initial maximum load. Diets high in sucrose and fat content have been associated with changes in calcium metabolism, and the results of the current study suggest that in immature vertebrae, a HFS diet may adversely affect vertebral body mechanical integrity and strength.
高脂肪和高蔗糖（HFS）饮食可能会导致葡萄糖不耐受、改变钙代谢，并导致骨矿化、骨骼发育及力学性能方面的缺陷。为了确定HFS饮食对快速生长的椎骨的力学和结构影响，将雌性斯普拉格-道利大鼠（8周龄）随机（2:1）分为两组，一组为对照组（n = 20），喂食低脂复合碳水化合物饮食；另一组为实验组（n = 10），喂食HFS饮食，持续10 - 12周。从椎弓根分离出第六腰椎椎体（L6），进行形态学测量，并在快速应变率下进行压缩测试，同时将其浸入温热（37摄氏度）的等渗生理缓冲溶液中。HFS组和对照组之间体重无显著差异；然而，HFS组L6的横截面积、长度和体积均显著小于对照组。HFS组L6的力学性能也显著较低，包括初始最大载荷、初始最大载荷时的能量以及初始最大载荷时的应变能密度。高蔗糖和高脂肪含量的饮食与钙代谢变化有关，本研究结果表明，在未成熟的椎骨中，HFS饮食可能会对椎体的力学完整性和强度产生不利影响。
