Kasuya M, Itoi M, Kobayashi S, Sunaga H, Suzuki K T
Eye Institute for the Cataract Foundation, Tokyo, Japan.
Exp Eye Res. 1992 Jan;54(1):49-53. doi: 10.1016/0014-4835(92)90068-4.
Sulphur-containing compounds in the lens were studied in relation to galactose cataract formation. Female Wistar rats were fed a 35% galactose diet and the changes in lens sulphur concentration and its distribution on a gel filtration column were compared with age-related changes. Concentration of sulphur in the whole lens decreased with time. A low constant level was attained on the fifth day of the galactose diet. A decrease of sulphur concentration in the soluble fraction of the lens paralleled that of the whole lens which was correlated with the decrease of glutathione and taurine concentrations on a gel filtration column by high-performance liquid chromatography-inductively coupled argon plasma atomic emission spectrometry (HPLC-ICP). Concentration of magnesium in the lens decreased after the fifth day, while the ratio of sodium to potassium increased. These changes in sulphur-containing compounds and metals were observed prior to the onset of cataract formation.
研究了晶状体中含硫化合物与半乳糖性白内障形成的关系。给雌性Wistar大鼠喂食35%的半乳糖饮食，并将晶状体硫浓度的变化及其在凝胶过滤柱上的分布与年龄相关变化进行比较。整个晶状体中的硫浓度随时间降低。在半乳糖饮食的第五天达到低恒定水平。晶状体可溶部分中硫浓度的降低与整个晶状体的降低平行，这与通过高效液相色谱-电感耦合氩等离子体原子发射光谱法（HPLC-ICP）在凝胶过滤柱上谷胱甘肽和牛磺酸浓度的降低相关。晶状体中的镁浓度在第五天后降低，而钠钾比增加。这些含硫化合物和金属的变化在白内障形成之前就已观察到。
