Janatipour M, Naumov Y, Ando A, Sugimura K, Okamoto N, Tsuji K, Abe K, Inoko H
Department of Transplantation II, Tokai University School of Medicine, Isehara, Japan.
Immunogenetics. 1992;35(4):272-8. doi: 10.1007/BF00166833.
Taking advantage of five mouse genomic or cDNA probes [KE5(probe 14), KE4 (probe 11), KE3 (probe 7), KE2 (probe 5), and SET] mapped on the H-2K region in mouse, we have identified and localized homologues of these five genes in the human major histocompatibility complex region (HKE5, HKE4, HKE3, HKE2, and HSET, respectively). Cosmid cloning and pulsed field gel electrophoresis analyses indicated that a human homologous gene, HKE5, is located 10 kilobases (kb) centromeric of the alpha 2 (XI) collagen (COL11A2) gene followed by HKE4. HKE3, closely linked to HKE2, is located 170 kb centromeric of HKE4. Furthermore, HSET is located 50 kb centromeric of HKE2. This gene organization outside the DP subregion is completely identical to that of the mouse H-2K region centromeric of I-Pb3, a mouse homologue of the DPB gene, except the lack of genes corresponding to the H-2K and -K2 genes in human.
利用定位在小鼠H-2K区域的5种小鼠基因组或cDNA探针[KE5（探针14）、KE4（探针11）、KE3（探针7）、KE2（探针5）和SET]，我们已在人类主要组织相容性复合体区域鉴定并定位了这5个基因的同源物（分别为HKE5、HKE4、HKE3、HKE2和HSET）。黏粒克隆和脉冲场凝胶电泳分析表明，人类同源基因HKE5位于α2（XI）胶原蛋白（COL11A2）基因着丝粒侧10千碱基（kb）处，其后是HKE4。与HKE2紧密连锁的HKE3位于HKE4着丝粒侧170 kb处。此外，HSET位于HKE2着丝粒侧50 kb处。DP亚区域外的这种基因组织与小鼠I-Pb3（DPB基因的小鼠同源物）着丝粒侧的小鼠H-2K区域完全相同，只是人类缺乏与H-2K和-K2基因相对应的基因。
