Braine H G, Kickler T S, Charache P, Ness P M, Davis J, Reichart C, Fuller A K
Transfusion. 1986 Jul-Aug;26(4):391-3. doi: 10.1046/j.1537-2995.1986.26486262752.x.
In a 3-month period four episodes of bacterial sepsis related to contaminated random donor platelet concentrates were observed. Evaluation incriminated extended (5 or more days) platelet storage. To determine if platelets stored for longer periods were likely to have greater bacterial contamination, platelet concentrates were inoculated with bacteria and daily quantitative bacteriology was performed. In vitro studies using polyolefin bags indicated contamination could occur with a single organism of Staphylococcus epidermidis. By 72 hours, platelet concentrates contained from 10(3) to 10(8) organisms per 0.1 ml depending on the inoculum size. By 6 days all inoculated units contained 10(7) to 10(9) organisms per 0.1 ml.
在3个月的时间里，观察到4起与受污染的随机供者血小板浓缩物相关的细菌性败血症事件。评估认为是延长（5天或更长时间）的血小板储存所致。为了确定储存时间较长的血小板是否更有可能受到细菌污染，将细菌接种到血小板浓缩物中，并进行每日定量细菌学检测。使用聚烯烃袋进行的体外研究表明，表皮葡萄球菌单一菌种即可造成污染。到72小时时，根据接种量的大小，每0.1毫升血小板浓缩物中含有10³至10⁸个细菌。到第6天时，所有接种的单位每0.1毫升含有10⁷至10⁹个细菌。
