HESS E L, COBURE A
J Gen Physiol. 1950 May 20;33(5):511-23. doi: 10.1085/jgp.33.5.511.
Experimental evidence is presented that the intrinsic viscosity of solutions of mixed proteins obeys the additive equation See PDF for Equation. The datum serves to characterize the system, and combined with other analytical techniques and fractionation procedures, enables one to analyze and characterize subfractions. The plasmas and sera of clinically "normal" individuals give intrinsic viscosity values agreeing with calculated values. The intrinsic viscosity values for pathological plasmas and sera in all cases are greater than normal and reflect the augmented levels of those proteins fibrinogen, alpha(2)-globulins, and gamma-globulins occurring in the pathological state. The method is readily adaptable to routine clinical use and furnishes a measure of the departure from normal of protein levels in serum and plasma.
实验证据表明，混合蛋白质溶液的特性粘度服从加和方程（见PDF中的方程）。该数据用于表征系统，并与其他分析技术和分级分离程序相结合，使人们能够分析和表征亚组分。临床“正常”个体的血浆和血清给出的特性粘度值与计算值一致。在所有情况下，病理性血浆和血清的特性粘度值均高于正常水平，反映了病理状态下纤维蛋白原、α(2)-球蛋白和γ-球蛋白等蛋白质水平的升高。该方法易于应用于常规临床使用，并提供了一种衡量血清和血浆中蛋白质水平偏离正常的指标。
