Gu Tingyue, Syu Mei-Jywan
Department of Chemical Engineering, Ohio University, Athens, Ohio 45701, USA.
Biotechnol Prog. 2004 Sep-Oct;20(5):1460-6. doi: 10.1021/bp049776k.
Immobilized cells are widely used in bioconversions to produce biological products as well as in wastewater treatment such as solvent removal from wastewater streams. In this work, a rate model is proposed to simulate this kind of process in an axial-flow fixed-bed column packed with porous particles containing immobilized cells. The transient model considered various mass transfer mechanisms including axial dispersion, interfacial film mass transfer, and intraparticle diffusion. Cell death in the immobilized cell system was also considered. Effects of various parameters such as kinetic constants and mass transfer parameters were studied. Operational situations such as feed fluctuation flow rate increase and two columns in series were also investigated. The model can be used to study the behavior and characteristics of immobilized cell columns in order to perform scale-up predictions of effluent profiles and for the purpose of process optimization.
固定化细胞广泛应用于生物转化以生产生物产品，以及废水处理，如从废水流中去除溶剂。在这项工作中，提出了一个速率模型来模拟在填充有含固定化细胞的多孔颗粒的轴流固定床柱中的此类过程。瞬态模型考虑了各种传质机制，包括轴向扩散、界面膜传质和颗粒内扩散。还考虑了固定化细胞系统中的细胞死亡。研究了各种参数（如动力学常数和传质参数）的影响。还研究了诸如进料流量波动增加和两列串联等操作情况。该模型可用于研究固定化细胞柱的行为和特性，以便对流出物分布进行放大预测并用于过程优化。
