Uzal Francisco A, Woods Leslie, Stillian Michelle, Nordhausen Robert, Read Deryck H, Van Kampen Hanna, Odani Jenee, Hietala Sharon, Hurley E J, Vickers Mary L, Gard Sharon M
California Animal Health and Food Safety Laboratory System, San Bernardino Branch, School of Veterinary Medicine, University of California, Davis, San Bernardino, CA 92408, USA.
J Vet Diagn Invest. 2004 Sep;16(5):478-84. doi: 10.1177/104063870401600523.
Three outbreaks of late-gestation abortions in does and ulcerative posthitis in bucks, associated with caprine herpes virus-1 (CHV-1), in California are described. In herd A, 10 of 17 does aborted in a 7-day period, whereas in herd B, 4 of 130 does aborted in a 45-day period and in herd C, 100 of 300 does aborted in a 3-week period. Most fetuses had multifocal pinpoint depressed foci with a zone of hyperemia on external and cut surfaces of the kidneys, liver, lungs, and adrenal glands. Histologically, scattered multifocal areas of necrosis with mild neutrophilic infiltrate were observed in kidneys, brain, liver, adrenal glands, and lungs of most fetuses of the 3 herds. Large amphophilic intranuclear inclusion bodies, which displaced the chromatin, were observed in cells within and around the necrotic foci in kidneys and adrenal glands. Particles 85-113 nm in size with morphology compatible with herpes virus were observed in the nuclei of these cells when examined by electron microscopy. Irregular, shallow, red ulcers were observed in the prepuce of 1 buck from herd C. Prepuce biopsies from this animal had necrosis of the superficial mucosal epithelium and severe submucosal lymphoplasmocytic infiltrates. Large intranuclear amphophilic inclusion bodies were observed in most cells of the stratum spinosum of the preputial epithelium, but no viral particles were observed in these cells. Caprine herpes virus-1 was isolated from tissue pools of fetuses from the 3 herds but not from prepuce biopsies. Positive results were obtained when tissues of a fetus from herd C were processed by a polymerase chain reaction technique to amplify the amino terminus of the glycoprotein C gene of CHV-1. Sera from aborted does from herds B and C and from the 3 bucks from herd C had high antibody titers to CHV-1. The results presented here support the hypothesis that the male goat is involved in the transmission of CHV-1. However, other forms of transmission cannot be ruled out.
本文描述了加利福尼亚州发生的三起与山羊疱疹病毒1型（CHV-1）相关的妊娠后期母羊流产和公羊溃疡性包皮炎疫情。在A群中，17只母羊中有10只在7天内流产；在B群中，130只母羊中有4只在45天内流产；在C群中，300只母羊中有100只在3周内流产。大多数胎儿的肾脏、肝脏、肺和肾上腺的外表和切面有多处微小的凹陷病灶，并伴有充血带。组织学检查发现，这三个羊群中大多数胎儿的肾脏、大脑、肝脏、肾上腺和肺有散在的多灶性坏死区域，并伴有轻度嗜中性粒细胞浸润。在肾脏和肾上腺坏死灶内及周围的细胞中观察到大型嗜酸性核内包涵体，其使染色质移位。用电镜检查时，在这些细胞的细胞核中观察到大小为85-113纳米、形态与疱疹病毒相符的颗粒。在C群的1只公羊的包皮中观察到不规则、浅红色的溃疡。该动物的包皮活检显示浅表黏膜上皮坏死和严重的黏膜下淋巴浆细胞浸润。在包皮上皮棘层的大多数细胞中观察到大型核内嗜酸性包涵体，但在这些细胞中未观察到病毒颗粒。从这三个羊群的胎儿组织池中分离出了山羊疱疹病毒1型，但未从包皮活检组织中分离出。当用聚合酶链反应技术处理C群一只胎儿的组织以扩增CHV-1糖蛋白C基因的氨基末端时，获得了阳性结果。B群和C群流产母羊以及C群3只公羊的血清对CHV-1有高抗体滴度。此处呈现的结果支持雄性山羊参与CHV-1传播的假说。然而，不能排除其他传播形式。
