[使用小鼠淋巴瘤tk基因突变试验评估长春新碱和秋水仙碱的遗传毒性]
[Evaluation of the genotoxicity of vincristine and colchicine using mouse lymphoma tk mutation assay].
作者信息
Wang Yi-jing, Zhang Li-shi
机构信息
West China School of Public Health, Sichuan University, Chengdu 610041, China.
出版信息
Wei Sheng Yan Jiu. 2006 Mar;35(2):179-81.
OBJECTIVE
To evaluate the genotoxicity of two mitotic inhibitors vincristine and colchicine, and to compare the detecting sensitivity of tk gene mutation assay with short-and long-term treatment by them.
METHODS
Tk gene mutation assay was performed by microwell method with L5178Y mouse lymphoma cell line after exposing to vincristine and colchicine for 3 h and 24 h respectively, and the results of two different treating time were compared.
RESULTS
The two chemicals didn't show positive responses in mouse lymphoma assay (MLA) with 3h treatment, but they could induce tk mutation frequency increase 1.6 to 4.8 and 2.1 to 6.2 times higher to spontaneous MF respectively after continuous treatment for 24 h.
CONCLUSION
Vincristine and colchicine had mutagenic effect on tk gene with long-term treatment, which indicated that the detecting sensitivity of MLA could be enhanced by long-term treatment.
目的
评估两种有丝分裂抑制剂长春新碱和秋水仙碱的遗传毒性，并比较tk基因突变试验对它们短期和长期处理的检测敏感性。
方法
分别用长春新碱和秋水仙碱处理L5178Y小鼠淋巴瘤细胞系3小时和24小时后，采用微孔板法进行tk基因突变试验，并比较两种不同处理时间的结果。
结果
两种化学物质在处理3小时的小鼠淋巴瘤试验（MLA）中未显示阳性反应，但在连续处理24小时后，它们可分别诱导tk突变频率比自发突变频率增加1.6至4.8倍和2.1至6.2倍。
结论
长春新碱和秋水仙碱长期处理对tk基因有诱变作用，这表明长期处理可提高MLA的检测敏感性。
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