Liu Sheng-xue, Cao Jia, Yuan Jian, Huang Ping, Shua Pei-qiang, Honma Masamitsu
Department of Health Toxicology, Third Military Medical University, Chongqing 400038, China.
Zhongguo Zhong Yao Za Zhi. 2003 Oct;28(10):954-7.
To investigate the effects of total alkaloids of Tripterygium hypoglaucum Hutch (THH) on the tk gene of mouse lymphoma cells.
L5178Y cells were infected with total alkaloids of THH with different concentrations and put into single-cell wells at different time phases. Then the numbers of positive wells were counted and the cell plating efficiency, relative suspension growth and mutation frequency were determined.
Total alkaloids of THH (0.1-2.0 g x L(-1)) induced tk locus mutation with mutation frequency 2-9 times higher than that of spontaneous mutation frequency of L5178Y cells. There were two different phenotypes of mutation colonies, large colony and small colony, but the main colony was large colony. This phenomenon might be related with the mutagenesis of THH.
Total alkaloids of THH can exert toxicity and mutagenic effects on tk gene in L5178Y cells, and there may be range limit in gene mutation.
研究昆明山海棠总生物碱（THH）对小鼠淋巴瘤细胞tk基因的影响。
用不同浓度的THH总生物碱感染L5178Y细胞，并在不同时间阶段接种于单细胞孔中。然后计数阳性孔的数量，并测定细胞接种效率、相对悬浮生长和突变频率。
THH总生物碱（0.1 - 2.0 g·L⁻¹）诱导tk位点突变，突变频率比L5178Y细胞自发突变频率高2 - 9倍。有两种不同表型的突变集落，大集落和小集落，但主要集落为大集落。这种现象可能与THH的诱变作用有关。
THH总生物碱可对L5178Y细胞的tk基因产生毒性和诱变作用，且基因突变可能存在范围限制。
