Cristol J P, Canaud B, Damon M, Chavis C, Arnoux B, Mion C
Department of Nephrology, Hospital Lapeyronie, Montpellier, France.
Prostaglandins Leukot Essent Fatty Acids. 1992 Jan;45(1):37-42. doi: 10.1016/0952-3278(92)90100-w.
Platelet activating factor (PAF) production and platelet-lipoxygenase activity were studied during hemodialysis (HD) with cuprophane membranes. Six patients were treated with first-use dialyzers (FU), and 6 patients with reused dialyzers (RU). In a random and double-blind design, 2 HD were performed for each patient, with or without BN 52021 pretreatment, a selective PAF antagonist. Platelet and leukocyte counts were performed before pretreatment and 30 min before HD starting (T-30), at the beginning of HD (T0) and after 15 and 30 min of HD (T15, T30). PAF production was analyzed by direct phase HPLC. To determine platelet-lipoxygenase activity, 12-HETE was detected by reverse phase high performance liquid chromatography (HPLC) after blood stimulation by the ionophore A23187. In the FU group, PAF and 12-HETE were produced during the first 30 min of HD. After BN 52021 pretreatment, PAF production was suppressed and platelet-lipoxygenase activity reduced. In the RU group, neither PAF nor 12-HETE production occurred, and BN 52021 had no effect. We conclude that PAF, which was involved in both platelet and leukocyte activation that occurred during hemodialysis, can be considered as a bio-incompatibility marker.
使用铜仿膜进行血液透析（HD）期间，研究了血小板活化因子（PAF）的产生及血小板脂氧合酶活性。6例患者使用首次使用的透析器（FU）治疗，6例患者使用复用透析器（RU）治疗。采用随机双盲设计，每位患者进行2次血液透析，一次进行或不进行BN 52021预处理（一种选择性PAF拮抗剂）。在预处理前以及血液透析开始前30分钟（T-30）、血液透析开始时（T0）以及血液透析15分钟和30分钟后（T15、T30）进行血小板和白细胞计数。通过直接相高效液相色谱法分析PAF的产生。为测定血小板脂氧合酶活性，在用离子载体A23187刺激血液后，通过反相高效液相色谱法（HPLC）检测12-羟基二十碳四烯酸（12-HETE）。在FU组中，血液透析的前30分钟内产生了PAF和12-HETE。BN 52021预处理后，PAF的产生受到抑制，血小板脂氧合酶活性降低。在RU组中，未产生PAF和12-HETE，且BN 52021无作用。我们得出结论，PAF参与了血液透析期间发生的血小板和白细胞活化，可被视为生物不相容性标志物。
