Davoodpour Padideh, Bergström Mats, Landström Maréne
Ludwig Institute for Cancer Research, Box 595, Biomedical Center, Uppsala, Sweden.
Nucl Med Biol. 2004 Oct;31(7):867-74. doi: 10.1016/j.nucmedbio.2004.03.015.
The purpose of this study was to investigate the potential use of PET in vivo to record cytotoxic effects of 2-methoxyestradiol (2-ME), an endogenous metabolite of 17beta-estradiol. The anti-proliferative and pro-apoptotic effects of 2-ME on human prostate cancer cell (PC3) aggregates in vitro, were correlated with the uptake of fluoro-deoxy-D-glucose, FMAU and choline labelled with 18F, 11C, or 3H. 2-ME clearly reduced growth of PC3 aggregates and induced apoptosis in a dose-dependent manner. However, the uptake of the putative proliferation markers 11C-FMAU or 3H-choline failed to record the growth inhibitory effects of 2-ME on PC3 cell aggregates. The uptake of 18F-FDG was used as a marker for effects on cellular metabolism and also failed to show any dose-dependent effects in PC3 aggregates. The use of these PET-tracers in vivo is therefore not recommended in order to evaluate the cytotoxic effects of 2-ME on human prostate cancer cells.
本研究的目的是探讨正电子发射断层扫描（PET）在体内记录17β-雌二醇的内源性代谢物2-甲氧基雌二醇（2-ME）细胞毒性作用的潜在用途。2-ME对人前列腺癌细胞（PC3）聚集体的体外抗增殖和促凋亡作用，与用18F、11C或3H标记的氟代脱氧葡萄糖、氟代甲基阿糖脲（FMAU）和胆碱的摄取相关。2-ME明显降低了PC3聚集体的生长，并以剂量依赖的方式诱导细胞凋亡。然而，假定的增殖标志物11C-FMAU或3H-胆碱的摄取未能记录2-ME对PC3细胞聚集体的生长抑制作用。18F-FDG的摄取用作细胞代谢影响的标志物，在PC3聚集体中也未显示任何剂量依赖性效应。因此，不建议在体内使用这些PET示踪剂来评估2-ME对人前列腺癌细胞的细胞毒性作用。
