Comi Anne M, Weisz Catherine J C, Highet Bridget H, Johnston Michael V, Wilson Mary Ann
Department of Neurology, Johns Hopkins University School of Medicine, 600 N. Wolfe Street, Baltimore, MD 21287, USA.
Pediatr Neurol. 2004 Oct;31(4):254-7. doi: 10.1016/j.pediatrneurol.2004.04.010.
Ischemic brain injury from stroke is an important cause of disability in infants and children, but current experimental models for the disorder are complex. These preparations require occlusion of small intracerebral vessels or common carotid artery ligation combined with exposure to reduced levels of oxygen. Unilateral carotid artery ligation alone was sufficient to cause brain injury in more than 70% of 12-day-old CD1 mice. Using a blinded behavioral rating scale of seizure activity in mice, a direct, highly significant correlation between the severity of seizures over the 4-hour period after ligation and the severity of histologic brain injury 7 days later (Spearman's rho = 0.835, P < 0.001) was documented. This study presents the first model of stroke in immature mice produced by unilateral carotid artery ligation alone, and the first to demonstrate a clear correlation between acute ischemia-induced seizures and brain injury. This new model should be useful for examining the pathogenesis of stroke in the immature brain and the potential contribution of seizures to final outcome.
中风导致的缺血性脑损伤是婴幼儿残疾的重要原因，但目前针对该病症的实验模型较为复杂。这些实验准备需要闭塞脑内小血管或结扎颈总动脉，并结合低氧暴露。仅单侧颈动脉结扎就足以在70%以上的12日龄CD1小鼠中导致脑损伤。使用对小鼠癫痫活动的盲法行为评分量表，记录了结扎后4小时内癫痫发作的严重程度与7天后组织学脑损伤的严重程度之间存在直接、高度显著的相关性（Spearman秩相关系数=0.835，P<0.001）。本研究首次提出了仅通过单侧颈动脉结扎在未成熟小鼠中建立的中风模型，也是首个证明急性缺血性癫痫发作与脑损伤之间存在明确相关性的研究。这个新模型对于研究未成熟脑中风的发病机制以及癫痫发作对最终结果的潜在影响应该是有用的。
