MacFarlane S A, Gilmer D, Davies J W
Department of Virus Research, John Innes Institute, John Innes Centre for Plant Science Research, Norwich, United Kingdom.
Virology. 1992 Apr;187(2):829-31. doi: 10.1016/0042-6822(92)90488-b.
Clones have been constructed containing full-length cDNA copies of PEBV RNA1 and RNA2, flanked by the CaMV 35 S RNA promoter and the nopaline synthase terminator. The clones are infectious when inoculated onto Nicotiana benthamiana plants. Both the viral RNAs and the virus particles were identified in infected plants.
已构建出含有豌豆早枯病毒RNA1和RNA2全长cDNA拷贝的克隆，其两侧分别为花椰菜花叶病毒35S RNA启动子和胭脂碱合酶终止子。将这些克隆接种到本氏烟草植株上时具有感染性。在受感染的植株中鉴定出了病毒RNA和病毒粒子。
