Zugates Christopher T, Lee Tzumin
Department of Cell and Structural Biology, University of Illinois, Urbana, Illinois 61801, USA.
Curr Opin Neurobiol. 2004 Oct;14(5):647-53. doi: 10.1016/j.conb.2004.08.005.
Genetic mosaic techniques provide a powerful tool for dissecting gene function in the intricate genetic networks that underlie the formation and function of nervous systems. For instance, it is possible to make individual cells or groups of cells homozygous for mutations of interest at specific points during an organism's development. It is also possible to resolve lineage relationships and to characterize cellular morphology and connectivity. Current techniques for creating genetically mosaic organisms incorporate improved controls over clone induction, identification, and/or mosaic tissue characterization.
基因镶嵌技术为剖析构成神经系统并支撑其功能的复杂基因网络中的基因功能提供了强大工具。例如，在生物体发育的特定阶段，有可能使单个细胞或细胞群对感兴趣的突变纯合。还能够解析谱系关系，并对细胞形态和连接性进行表征。目前用于创建基因镶嵌生物的技术在克隆诱导、识别和/或镶嵌组织表征方面纳入了改进的控制措施。
