使用具有时空特异性的MADM进行体内克隆分析。

In Vivo Clonal Analysis Using MADM with Spatiotemporal Specificity.

作者信息

Peng Yuwei, Wang Efan, Shen Zhongfu, Shi Song-Hai, Ma Jian

机构信息

IDG/McGovern Institute of Brain Research, Tsinghua University, Beijing, People's Republic of China.

Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, People's Republic of China.

出版信息

Methods Mol Biol. 2025;2886:265-279. doi: 10.1007/978-1-0716-4310-5_13.

DOI:10.1007/978-1-0716-4310-5_13

PMID:39745645

Abstract

Mosaic analysis with double markers (MADM) is a powerful in vivo lineage tracing technique. It utilizes Cre recombinase-dependent interchromosomal recombination to restore the stable expression of two fluorescent proteins sparsely in individual dividing stem or progenitor cells and their progenies. Here, we describe the application of this technique for quantitative lineage analysis of radial glial progenitors in the developing mouse neocortex at the single-cell resolution.

摘要

双标记嵌合分析（MADM）是一种强大的体内谱系追踪技术。它利用依赖于Cre重组酶的染色体间重组，在单个分裂的干细胞或祖细胞及其后代中稀疏地恢复两种荧光蛋白的稳定表达。在这里，我们描述了该技术在单细胞分辨率下对发育中的小鼠新皮质中放射状胶质祖细胞进行定量谱系分析的应用。