Lima-Couy I, Cervero A, Bonilla-Musoles F, Pellicer A, Simón C
Department of Obstetrics and Gynecology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
Mol Hum Reprod. 2004 Nov;10(11):777-82. doi: 10.1093/molehr/gah115. Epub 2004 Oct 1.
The leptin system has been implicated in reproductive function, acting at endocrine and paracrine levels. Recently, deregulation of this gene family has been linked to endometrial changes caused by endometriosis. In the present study, we compare the expression of leptin receptor mRNA during the pre-receptive (LH+2) and receptive (LH+9) phases in the eutopic endometrium from patients with severe/moderate endometriosis (n = 30) versus fertile controls (n = 12). In each patient, two endometrial samples were obtained at LH+2 and LH+9 in their natural cycles. When real-time quantitative fluorescent PCR was performed, an up-regulation of OB-RL and all the isoforms investigated was observed at LH+9 versus LH+2 in patients with and without endometriosis. However, no difference was found in the expression pattern of the total leptin receptor OB-RT, or in its long OB-RL and soluble HuB219.3 forms when the eutopic endometria of patients with severe/moderate endometriosis and fertile controls were compared. By means of in situ hybridization, total leptin receptor mRNA was localized in the luminal epithelium and the glands of the endometrium. The immunohistochemical analysis of the long form of leptin receptor was also performed in order to confirm these findings at the protein level. Finally, we have also shown similar leptin mRNA expression in both the control group and patients with endometriosis. In conclusion, we have not identified differences in the endometrial expression and localization of leptin and the leptin receptor when comparing the eutopic endometrium of women with severe/moderate endometriosis and fertile controls.
瘦素系统已被认为与生殖功能有关，在内分泌和旁分泌水平发挥作用。最近，该基因家族的失调与子宫内膜异位症引起的子宫内膜变化有关。在本研究中，我们比较了重度/中度子宫内膜异位症患者（n = 30）与正常生育对照组（n = 12）在位内膜在接受前期（LH+2）和接受期（LH+9）时瘦素受体mRNA的表达。在每个患者的自然周期中，于LH+2和LH+9时获取两份子宫内膜样本。进行实时定量荧光PCR时，在LH+9时，无论有无子宫内膜异位症，OB-RL及所有研究的异构体均较LH+2时上调。然而，比较重度/中度子宫内膜异位症患者与正常生育对照组的在位内膜时，总瘦素受体OB-RT及其长型OB-RL和可溶性HuB219.3形式的表达模式未发现差异。通过原位杂交，总瘦素受体mRNA定位于子宫内膜的腔上皮和腺体。还进行了瘦素受体长型的免疫组化分析，以在蛋白质水平证实这些发现。最后，我们还显示对照组和子宫内膜异位症患者的瘦素mRNA表达相似。总之，比较重度/中度子宫内膜异位症女性与正常生育对照组的在位内膜时，我们未发现瘦素及其受体在子宫内膜表达和定位上的差异。
