用于检测贝类中诺如病毒的逆转录增强型聚合酶链反应
Reverse transcription-booster PCR for detection of noroviruses in shellfish.
作者信息
De Medici Dario, Croci Luciana, Suffredini Elisabetta, Toti Laura
机构信息
Laboratorio Alimenti, Istituto Superior di Sanità, Rome, Italy.
出版信息
Appl Environ Microbiol. 2004 Oct;70(10):6329-32. doi: 10.1128/AEM.70.10.6329-6332.2004.
Abstract
The methods commonly used for norovirus (NV) detection are based on reverse transcription-PCR (RT-PCR) followed by confirmation of the amplified sequence. To increase sensitivity, an RT-booster PCR was developed. The proposed method showed an increase in sensitivity at least 2 log units for all the NV strains tested compared with the standard RT-PCR method. Higher sensitivity was confirmed in tests on experimentally and naturally contaminated shellfish.
摘要
用于诺如病毒（NV）检测的常用方法是基于逆转录聚合酶链反应（RT-PCR），随后对扩增序列进行确认。为了提高灵敏度，开发了一种RT增强型PCR。与标准RT-PCR方法相比，所提出的方法对所有测试的NV毒株的灵敏度至少提高了2个对数单位。在对实验性和自然污染的贝类进行的测试中证实了更高的灵敏度。
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