Chatterjee S N, Chaudhuri Keya
Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Sector-1, Calcutta-700 064, India.
Biochim Biophys Acta. 2004 Oct 14;1690(2):93-109. doi: 10.1016/j.bbadis.2004.06.006.
An account of our up to date knowledge of the genetics of biosynthesis of Vibrio cholerae lipopolysaccharide (LPS) is presented in this review. While not much information is available in the literature on the genetics of biosynthesis of lipid A of V. cholerae, the available information on the characteristics and proposed functions of the corepolysaccharide (core-PS) biosynthetic genes is discussed. The genetic organizations encoding the O-antigen polysaccharides (O-PS) of V. cholerae of serogroups O1 and O139, the disease causing ones, have been described along with the putative functions of the different constituent genes. The O-PS biosynthetic genes of some non-O1, non-O139 serogroups, particularly the serogroups O37 and O22, and their putative functions have also been discussed briefly. In view of the importance of the serogroup O139, the origination of the O139 strain and the possible donor of the corresponding O-PS gene cluster have been analyzed with a view to having knowledge of (i) the mode of evolution of different serogroups and (ii) the possible emergence of pathogenic strain(s) belonging to non-O1, non-O139 serogroups. The unsolved problems in this area of research and their probable impact on the production of an effective cholera vaccine have been outlined in conclusion.
本综述介绍了我们目前对霍乱弧菌脂多糖（LPS）生物合成遗传学的认识。虽然关于霍乱弧菌脂质A生物合成遗传学的文献资料不多，但本文讨论了关于核心多糖（core-PS）生物合成基因的特征和推测功能的现有信息。本文描述了编码引起疾病的O1和O139血清群霍乱弧菌O抗原多糖（O-PS）的基因组织，以及不同组成基因的推测功能。一些非O1、非O139血清群，特别是O37和O22血清群的O-PS生物合成基因及其推测功能也进行了简要讨论。鉴于O139血清群的重要性，分析了O139菌株的起源和相应O-PS基因簇的可能供体，以便了解（i）不同血清群的进化模式，以及（ii）属于非O1、非O139血清群的致病菌株的可能出现情况。最后概述了该研究领域中尚未解决的问题及其对有效霍乱疫苗生产的可能影响。
