Iida M, Watanabe K, Tsurufuji M, Takaishi K, Iizuka Y, Tsurufuji S
Institute of Cytosignal Research Inc., Tokyo, Japan.
Infect Immun. 1992 Apr;60(4):1268-72. doi: 10.1128/iai.60.4.1268-1272.1992.
Rat cytokine-induced neutrophil chemoattractant (CINC), which is a counterpart of human gro and belongs to the interleukin-8 family, has been quantified by a new sandwich enzyme-linked immunosorbent assay. Administration of lipopolysaccharide (LPS) into an air pouch performed by subcutaneous injection of air caused inflammation and severe neutrophil infiltration. After the LPS injection, changes in the concentration of CINC/gro, chemotactic activity, and the number of neutrophils in the air pouch exudate were determined. The chemotactic activity of neutrophils was augmented before practical neutrophil infiltration. More than half of the chemotactic activity was neutralized by the antisera. The time kinetics of the level of CINC/gro coincided with the changes in chemotactic activity. The maximal level of rat CINC/gro was 85 ng/ml, which is sufficient to cause neutrophil migration in vitro and in vivo as described previously. These data suggest that rat CINC/gro is a functional chemoattractant for neutrophils in LPS-induced inflammation in rats.
大鼠细胞因子诱导的中性粒细胞趋化因子（CINC），它是人类生长调节致癌基因α（gro）的对应物，属于白细胞介素-8家族，已通过一种新的夹心酶联免疫吸附测定法进行了定量。通过皮下注射空气在气袋中给予脂多糖（LPS）会引起炎症和严重的中性粒细胞浸润。在注射LPS后，测定了气袋渗出液中CINC/gro的浓度变化、趋化活性以及中性粒细胞的数量。在实际的中性粒细胞浸润之前，中性粒细胞的趋化活性增强。超过一半的趋化活性被抗血清中和。CINC/gro水平的时间动力学与趋化活性的变化一致。大鼠CINC/gro的最大水平为85 ng/ml，这足以像先前描述的那样在体外和体内引起中性粒细胞迁移。这些数据表明，大鼠CINC/gro是LPS诱导的大鼠炎症中中性粒细胞的一种功能性趋化因子。
