Konno S, Tsurufuji S
Br J Pharmacol. 1983 Oct;80(2):269-77. doi: 10.1111/j.1476-5381.1983.tb10030.x.
Induction of an experimental inflammation of the air-pouch type with the aid of zymosan (known to activate the alternative pathway of the complement system) was carried out in an attempt to induce a reproducible inflammatory model suitable for quantitative studies. Rats were injected subcutaneously with 8 ml of air on the dorsal surface to make an air-pouch, followed 24h later by 4 ml of 1.6% (w/v) zymosan suspension. This induced inflammatory responses. Treatment with zymosan suspension provoked exudation of fluid, accumulation of polymorphnuclear leukocytes (PMN) in the pouch and the development of granulation tissue as a wall of the pouch. Approximately half of the PMN in the pouch formed a characteristic layer of aggregated cells sticking onto the inner surface of the pouch wall. They were counted after being completely disaggregated by means of incubation with trypsin. In preliminary experiments with potential anti-inflammatory drugs, local application of a novel anti-complementary agent, K-76COONa, inhibited leukocyte accumulation in the pouch, whereas the potent anti-inflammatory agent, dexamethasone, was ineffective. By contrast, exudation of the pouch fluid was effectively inhibited by dexamethasone but not by K-76COONa.
为了诱导出适合定量研究的可重复性炎症模型，借助酵母聚糖（已知其可激活补体系统的替代途径）进行了气袋型实验性炎症的诱导。在大鼠背部皮下注射8毫升空气以形成气袋，24小时后再注射4毫升1.6%（重量/体积）的酵母聚糖悬液。这引发了炎症反应。用酵母聚糖悬液处理会引起液体渗出、多形核白细胞（PMN）在气袋中积聚以及肉芽组织作为气袋壁的形成。气袋中大约一半的PMN形成了一层聚集细胞的特征层，附着在气袋壁的内表面。在用胰蛋白酶孵育使其完全分散后对它们进行计数。在使用潜在抗炎药物的初步实验中，新型抗补体剂K-76COONa的局部应用抑制了气袋中白细胞的积聚，而强效抗炎剂地塞米松则无效。相比之下，地塞米松有效地抑制了气袋液体的渗出，但K-76COONa没有。
