Oquendo P, Alberta J, Wen D Z, Graycar J L, Derynck R, Stiles C D
Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.
J Biol Chem. 1989 Mar 5;264(7):4133-7.
A full length cDNA clone of the platelet-derived growth factor-inducible KC gene has been isolated, sequenced, and expressed in COS cells. Both sequence analysis and expression studies indicate that KC encodes a secretory protein. Sequence analysis shows that, furthermore, the protein encoded by KC belongs to a growing superfamily of inducible proteins with a common ancestral linkage to the platelet alpha-granule proteins, platelet factor 4, and connective tissue-activating peptide III. A computer-generated phylogenetic tree documents interrelationships between KC and six additional members of this peptide superfamily. The KC gene is, in all probability, the murine homologue of a human gene termed "gro." By extension, the KC protein is the murine counterpart of the protein encoded by the gro gene. The gro protein corresponds to a factor described as "melanoma growth-stimulating activity."
已分离出血小板衍生生长因子诱导的KC基因的全长cDNA克隆，对其进行了测序，并在COS细胞中进行了表达。序列分析和表达研究均表明，KC编码一种分泌蛋白。此外，序列分析显示，KC编码的蛋白属于一个不断扩大的诱导蛋白超家族，该家族与血小板α-颗粒蛋白、血小板因子4和结缔组织激活肽III具有共同的祖先联系。计算机生成的系统发育树记录了KC与该肽超家族的其他六个成员之间的相互关系。KC基因很可能是一个名为“gro”的人类基因的小鼠同源物。由此推断，KC蛋白是gro基因编码的蛋白的小鼠对应物。gro蛋白对应于一种被描述为“黑色素瘤生长刺激活性”的因子。
