Sonoda Hiromichi, Yamamoto Kazuo, Kushima Ryoji, Okabe Hidetoshi, Tani Tohru
Department of Surgery, Shiga University of Medical Science, Seta, Otsu, Shiga, Japan.
J Surg Oncol. 2004 Nov 1;88(2):63-70. doi: 10.1002/jso.20143.
The objectives of this study were to evaluate the clinical utility of MUC2-specific reverse transcriptase-polymerase chain reaction (RT-PCR) in gastric cancer patients and to compare MUC2-specific RT-PCR to carcinoembryonic antigen (CEA)-specific RT-PCR.
A total of 305 lymph nodes from 28 patients with gastric cancer were histologically examined and analyzed by MUC2 RT-PCR and CEA RT-PCR.
MUC2 and CEA were expressed in 17.1 and 7.0% of the 286 histologically negative lymph nodes, respectively. The detection rate of MUC2 was significantly higher than that of CEA (P < 0.01). MUC2 RT-PCR revealed no false positive results in control specimens. Lymph node micrometastases in pT1 gastric cancer were expressed only in perigastric lymph nodes near the tumor and were not detected in tumor less than 30 mm in patients with mucosal cancer.
MUC2-specific RT-PCR is a sensitive and specific method to detect lymph node micrometastases in gastric cancer patients. The distribution of lymph node micrometastases detected by this method may be useful in minimally invasive procedures for gastric cancer, especially pT1 cases.
本研究的目的是评估黏蛋白2（MUC2）特异性逆转录聚合酶链反应（RT-PCR）在胃癌患者中的临床应用价值，并将MUC2特异性RT-PCR与癌胚抗原（CEA）特异性RT-PCR进行比较。
对28例胃癌患者的305个淋巴结进行组织学检查，并通过MUC2 RT-PCR和CEA RT-PCR进行分析。
在286个组织学阴性的淋巴结中，MUC2和CEA的表达率分别为17.1%和7.0%。MUC2的检测率显著高于CEA（P < 0.01）。MUC2 RT-PCR在对照标本中未显示假阳性结果。pT1期胃癌的淋巴结微转移仅在肿瘤附近的胃周淋巴结中表达，在黏膜癌患者中肿瘤小于30 mm时未检测到。
MUC2特异性RT-PCR是检测胃癌患者淋巴结微转移的一种敏感且特异的方法。通过该方法检测到的淋巴结微转移分布可能有助于胃癌的微创手术，尤其是pT1期病例。
