Department of Pathology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China.
World J Gastroenterol. 2012 Feb 28;18(8):754-66. doi: 10.3748/wjg.v18.i8.754.
To investigate the effects of resistin-like molecule β (RELMβ) over-expression on the invasion, metastasis and angiogenesis of gastric cancer cells.
Human RELMβ encoding expression vector was constructed and transfected into the RELMβ lowly-expressed gastric cancer cell lines SGC-7901 and MKN-45. Gene expression was measured by Western blotting, reverse transcription polymerase chain reaction (PCR) and real-time quantitative PCR. Cell proliferation was measured by 2-(4,5-dimethyltriazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetry, colony formation and 5-ethynyl-20-deoxyuridine incorporation assays. The in vitro migration, invasion and metastasis of cancer cells were measured by cell adhesion assay, scratch assay and matrigel invasion assay. The angiogenic capabilities of cancer cells were measured by tube formation of endothelial cells.
Transfection of RELMβ vector into SGC-7901 and MKN-45 cells resulted in over-expression of RELMβ, which did not influence the cellular proliferation. However, over-expression of RELMβ suppressed the in vitro adhesion, invasion and metastasis of cancer cells, accompanied by decreased expression of matrix metalloproteinase-2 (MMP-2) and MMP-9. Moreover, transfection of RELMβ attenuated the expression of vascular endothelial growth factor and in vitro angiogenic capabilities of cancer cells.
Over-expression of RELMβ abolishes the invasion, metastasis and angiogenesis of gastric cancer cells in vitro, suggesting its potentials as a novel therapeutic target for gastric cancer.
研究抵抗素样分子β（RELMβ）过表达对胃癌细胞侵袭、转移和血管生成的影响。
构建人 RELMβ编码表达载体，并转染到 RELMβ 低表达的胃癌细胞系 SGC-7901 和 MKN-45 中。通过 Western blot、逆转录聚合酶链反应（PCR）和实时定量 PCR 检测基因表达。通过 2-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐比色法、集落形成和 5-乙炔基-20-脱氧尿苷掺入实验检测细胞增殖。通过细胞黏附实验、划痕实验和基质胶侵袭实验检测癌细胞的体外迁移、侵袭和转移能力。通过内皮细胞管形成实验检测癌细胞的血管生成能力。
RELMβ 载体转染 SGC-7901 和 MKN-45 细胞导致 RELMβ 过表达，但不影响细胞增殖。然而，RELMβ 的过表达抑制了癌细胞的体外黏附、侵袭和转移，同时降低了基质金属蛋白酶-2（MMP-2）和 MMP-9 的表达。此外，RELMβ 转染减弱了血管内皮生长因子的表达和癌细胞的体外血管生成能力。
RELMβ 的过表达可消除胃癌细胞的体外侵袭、转移和血管生成，提示其作为胃癌治疗的新靶点的潜力。
