Liang Gengtian, Cui Yonghua, Wei Minghui, Liu Zheng, Wang Chunfang
Department of Otorhinolaryngology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
Lin Chuang Er Bi Yan Hou Ke Za Zhi. 2004 Jul;18(7):411-3.
To study the protein expression of endotoxin, interleukin 1beta (IL-1beta) and regulated upon activation, normal T expressed and secreted (RANTES) in effusions of secretory otitis media and their roles in the pathogenesis of secretory otitis media.
Seventy-two effusion samples were taken from 53 patients with secretory otitis media by tympanocentesis. After swabs they were taken for bacteria culture. Limulus amebocyte lysate (LAL) test was used to quantify the content of endotoxin, radioimmunoassay to analyze the level of IL-1beta and enzyme-linked immunosorbent assay (ELISA) to detect the concentration of RANTES.
Endotoxin, IL-1beta and RANTES were detectable in 80.9%, 77.8% and 70.8% of middle ear effusion, with mean levels of (35.2 +/- 51.6) EU/ml, (1.10 +/- 0.84) microg/L and (0.52 +/- 0.43) microg/L respectively. All of them showed higher concentration in the mucoid-type effusions than those in the serous-type effusions (P<0.05). Higher levels of the endotoxin and RANTES (P<0.05) were found in longer course patients. The effusions with positive bacteria culture displayed more abundant endotoxin, IL-1beta and RANTES protein than those with negative culture. Significant correlations were found between endotoxin and IL-1beta (r = 0.74, P<0.01), also between IL-1beta and RANTES (r = 0.48, P<0.01).
Endotoxin, IL-1beta and RANTES may take part in maintaining the inflammatory response in the tympanic cavity and stimulating mucin secretion. It indicates that they play an important role in the immune pathogenesis of secretory otitis media.
研究分泌性中耳炎积液中内毒素、白细胞介素1β（IL-1β）及活化正常T细胞表达和分泌因子（RANTES）的蛋白表达情况及其在分泌性中耳炎发病机制中的作用。
对53例分泌性中耳炎患者行鼓膜穿刺抽取72份积液样本。抽取后用拭子进行细菌培养。采用鲎试剂法检测内毒素含量，放射免疫法分析IL-1β水平，酶联免疫吸附测定（ELISA）法检测RANTES浓度。
中耳积液中内毒素、IL-1β和RANTES的检出率分别为80.9%、77.8%和70.8%，平均水平分别为（35.2±51.6）EU/ml、（1.10±0.84）μg/L和（0.52±0.43）μg/L。三者在黏液型积液中的浓度均高于浆液型积液（P<0.05）。病程较长患者的内毒素和RANTES水平较高（P<0.05）。细菌培养阳性的积液中内毒素、IL-1β和RANTES蛋白含量比培养阴性的积液更丰富。内毒素与IL-1β之间（r = 0.74，P<0.01）以及IL-1β与RANTES之间（r = 0.48，P<0.01）均存在显著相关性。
内毒素、IL-1β和RANTES可能参与维持鼓室的炎症反应并刺激黏蛋白分泌。这表明它们在分泌性中耳炎的免疫发病机制中起重要作用。
