Jinnin T, Dake Y, Kusumoto T, Tabata T
Auris Nasus Larynx. 1985;12 Suppl 1:S97-9. doi: 10.1016/s0385-8146(85)80114-0.
It is known that bacterial endotoxin on the outer surface of most gram-negative bacteria (GNB) is not only biologically active material, but also the modulator of the immune response. The earlier experiment documented that the endotoxin of killed Haemophilus influenzae was responsible for induction of chronic otitis media with effusion (OME). Recent study by DeMaria et al. determined the endotoxin of human middle ear effusion (MEE) by means of limulus amebocyte lysate assay. It was concluded the endotoxin was present in a high percentage of the effusion in human tympanic cavities. The present study was undertaken to quantitatively determine the endotoxin in human MEE by use of chromogenic substrate technique which is more sensitive and accurate than limulus assay. Sixty samples of mucoid and serous effusion were subjected to chromogenic substrate method. It was revealed that the mucoid effusion showed a significantly high level of endotoxin at 282 pg/ml in average, though the serous effusion contained only 35.9 pg/ml. It is assumed that the bacterial endotoxin may greatly contribute to the pathogenesis of otitis media with mucoid effusion.
众所周知，大多数革兰氏阴性菌（GNB）外表面的细菌内毒素不仅是生物活性物质，也是免疫反应的调节剂。早期实验证明，灭活流感嗜血杆菌的内毒素是导致慢性分泌性中耳炎（OME）的原因。DeMaria等人最近的研究通过鲎试剂法测定了人中耳积液（MEE）中的内毒素。得出的结论是，内毒素在人鼓室积液中所占比例很高。本研究采用比鲎试剂法更灵敏、准确的显色底物技术，对人MEE中的内毒素进行定量测定。对60份黏液性和浆液性积液样本进行了显色底物法检测。结果显示，黏液性积液中内毒素平均水平显著较高，为282 pg/ml，而浆液性积液中仅含35.9 pg/ml。据推测，细菌内毒素可能在黏液性积液性中耳炎的发病机制中起很大作用。
