Abrams Stephanie B, Brock Guy N, Palettas Marilly, Bolner Michelle L, Moore-Sowers Tricia, Plunkett Greg A, Cole Lynette K, Diaz Sandra F, Lorch Gwendolen
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, 601 Vernon L. Tharp St., Columbus, OH, 43210, USA.
Center for Biostatistics, Department of Biomedical Informatics, The Ohio State University, 1800 Cannon Dr., Columbus, OH, 43210, USA.
Vet Dermatol. 2018 Dec;29(6):496-e167. doi: 10.1111/vde.12686. Epub 2018 Sep 3.
Limited information is available for dogs on threshold concentrations (TCs), and the protein composition of common allergenic extracts produced by different manufacturers.
HYPOTHESIS/OBJECTIVES: To characterize the protein heterogeneity of tree, grass, weed and mite allergens from different lots of allergenic extracts, and to determine intradermal TCs for healthy dogs using extracts from two manufacturers.
Twenty five privately owned, clinically healthy dogs and ten purpose-bred beagle dogs.
Protein concentration and heterogeneity of 11 allergens from two manufacturers were evaluated using a Bradford-style assay and SDS-PAGE. Intradermal testing was performed with 11 allergens from each company at four dilutions. Immediate reactions were subjectively scored (0 to 4+), and objectively measured (mm) and their percentage concordance evaluated. Model-based TCs were determined by fitting positive reactions (≥2+) at 15 min to generalized estimating equations.
Allergen extract protein quantity and composition varied within and between manufacturers despite sharing the same PNU/mL values. Model-based TCs of one weed, five trees, two grasses and a house dust mite were determined for extracts from Manufacturer 1 (M1), and for extracts of three weeds, three trees and two grasses from Manufacturer 2 (M2). Receiver operating characteristic curve analyses determined a percentage concordance of the objective and subjective measurements of 77.3% for M1 and 75% for M2 allergens.
Veterinary allergen extracts labelled as the same species and PNU/mL are not standardized; they show heterogeneity in composition and potency within and between manufacturers. Variability in extract content may require adjustment of intradermal testing concentrations.
关于犬类的阈值浓度（TCs）以及不同制造商生产的常见变应原提取物的蛋白质组成，可获取的信息有限。
假设/目标：表征不同批次变应原提取物中树木、草、杂草和螨虫变应原的蛋白质异质性，并使用来自两家制造商的提取物确定健康犬的皮内阈值浓度。
25只 privately owned、临床健康的犬和10只专门繁育的比格犬。
使用Bradford法和SDS - PAGE评估来自两家制造商的11种变应原的蛋白质浓度和异质性。对每家公司的11种变应原进行四种稀释度的皮内试验。对即时反应进行主观评分（0至4 +）、客观测量（毫米）并评估其百分比一致性。通过将15分钟时的阳性反应（≥2 +）拟合到广义估计方程来确定基于模型的阈值浓度。
尽管变应原提取物的每毫升蛋白氮单位（PNU/mL）值相同，但制造商内部和制造商之间的变应原提取物蛋白数量和组成存在差异。确定了制造商1（M1）提取物中一种杂草、五种树木、两种草和一种屋尘螨的基于模型的阈值浓度，以及制造商2（M2）提取物中三种杂草、三种树木和两种草的阈值浓度。受试者工作特征曲线分析确定，M1变应原的客观和主观测量的百分比一致性为77.3%，M2变应原的为75%。
标记为相同物种和PNU/mL的兽医变应原提取物未标准化；它们在制造商内部和制造商之间的组成和效力存在异质性。提取物含量的变异性可能需要调整皮内试验浓度。
