Serrano-Mollar A, Fernández-Zabalegui L, Bulbena O, Gelpí E, Closa D
Deparment of Experimental Pathology, IIBB-CSIC, IDIBAPS, c/ Rosselló 161, 7 08036 Barcelona, Spain.
Chem Biol Interact. 2004 Oct 15;149(2-3):117-23. doi: 10.1016/j.cbi.2004.07.003.
The Toxic Oil Syndrome (TOS) was a toxic epidemic disease, related to the consumption of rapeseed oil denatured with aniline that affected more than 20,000 people in Spain and resulted in more than 330 deaths after its sudden appearance in 1981. It has been reported that the fatty acid esters of 3-(N-phenylamino)-1,2-propanediol (PAP) have shown a strong association with TOS. These PAP-esters could be absorbed and metabolized in a similar way than phospholipids. This is of interest because some products of phospholipid metabolism are important mediators in downstream pathways involved in the regulation of different nuclear factors. In particular, phospholipase D activity is involved in the activation of c-fos. Thus, we have investigated the effect of different PAP-esters in the induction of c-fos in lung fibroblasts. Results indicate that PAP-esters rapidly induced the expression of c-fos in a dose-dependent manner. In addition, both butanol and propranolol prevent this induction pointing to the involvement of phospholipase D in this activation. These results suggest that deregulation of some nuclear factors such as AP-1 could be involved in the pathogenesis of TOS.
毒油综合征（TOS）是一种毒性流行疾病，与食用用苯胺变性的菜籽油有关，1981年突然出现后，在西班牙影响了2万多人，导致330多人死亡。据报道，3-（N-苯基氨基）-1,2-丙二醇（PAP）的脂肪酸酯与TOS有很强的关联。这些PAP酯可以以与磷脂类似的方式被吸收和代谢。这一点很重要，因为磷脂代谢的一些产物是参与调节不同核因子的下游途径中的重要介质。特别是，磷脂酶D活性参与c-fos的激活。因此，我们研究了不同PAP酯对肺成纤维细胞中c-fos诱导的影响。结果表明，PAP酯以剂量依赖的方式迅速诱导c-fos的表达。此外，丁醇和普萘洛尔都能阻止这种诱导，表明磷脂酶D参与了这种激活。这些结果表明，某些核因子如AP-1的失调可能与TOS的发病机制有关。
