Gallardo S, Cárdaba B, del Pozo V, de Andrés B, Cortegano I, Jurado A, Tramón P, Palomino P, Lahoz C
Department of Immunology, Fundación Jiménez Díaz, Madrid, Spain.
Toxicology. 1997 Mar 14;118(1):71-82. doi: 10.1016/s0300-483x(96)03585-8.
Toxic Oil Syndrome is a multisystemic disease that occurred in epidemic proportions in Spain in 1981 caused by the ingestion of rapeseed oil denatured with aniline. Several data implicate T cells in the pathogenesis of the disease. We evaluated the mechanisms of cytotoxicity in human lymphocytes of TOS-related products: aniline, 3-(N-phenylamino)-1,2-propanediol and its mono- and di-oleyl esters and eosinophilia myalgia-related product such as 3-(phenylamino)-L-alanine, which is chemically similar to 3-(N-phenylamino)-1,2-propanediol, and has been found in manufactured L-tryptophan. Our results show that only di-oleyl ester of 3-(N-phenylamino)-1,2-propanediol induces apoptosis in human lymphocytes, in a concentration and time-dependent way, confirmed by morphology, expression of phosphatidylserine in membrane and analysis of DNA degradation.
中毒性油综合征是一种多系统疾病，1981年在西班牙以流行形式出现，由摄入用苯胺变性的菜籽油引起。多项数据表明T细胞参与了该疾病的发病机制。我们评估了与中毒性油综合征相关的产品对人淋巴细胞的细胞毒性机制：苯胺、3-（N-苯基氨基）-1,2-丙二醇及其单油酸酯和二油酸酯，以及与嗜酸性粒细胞增多性肌痛相关的产品，如3-（苯氨基）-L-丙氨酸，其化学结构与3-（N-苯基氨基）-1,2-丙二醇相似，并且在生产的L-色氨酸中被发现。我们的结果表明，只有3-（N-苯基氨基）-1,2-丙二醇的二油酸酯以浓度和时间依赖性方式诱导人淋巴细胞凋亡，这通过形态学、膜上磷脂酰丝氨酸的表达以及DNA降解分析得到证实。
