蛙皮促胰液肽和雨蛙肽能刺激蛙皮的离子转运，但肠促胰液肽则不能。

Eledoisin and Kassinin, but not Enterokassinin, stimulate ion transport in frog skin.

作者信息

Lippe Claudio, Bellantuono Vito, Ardizzone Concetta, Cassano Giuseppe

机构信息

Dipartimento di Fisiologia Generale ed Ambientale, Università di Bari, Via Amendola 165/A, 70126 Bari, Italy.

出版信息

Peptides. 2004 Nov;25(11):1971-5. doi: 10.1016/j.peptides.2004.06.014.

DOI:10.1016/j.peptides.2004.06.014

PMID:15501529

Abstract

In frog skin, tachykinins stimulate the ion transport, estimated by measuring the short-circuit current (SCC) value, by interacting with NK1-like receptors. In this paper we show that Kassinin (NK2 preferring in mammals) increases the SCC, while Enterokassinin has no effect. Therefore, either 2 Pro residues or 1 Pro and 1 basic amino acid must be present in the part exceeding the C-terminal pentapeptide. Eledoisin (NK3 preferring in mammals) stimulation of SCC is reduced by CP99994 and SR48968 (NK1 and NK2 antagonists) and not affected by SB222200 (NK3 antagonist). None of the three antagonists affects Kassinin stimulation of SCC.

摘要

在蛙皮中，速激肽通过与NK1样受体相互作用刺激离子转运，离子转运通过测量短路电流（SCC）值来估算。在本文中，我们表明，蛙皮素（在哺乳动物中偏好NK2）可增加SCC，而肠蛙皮素则无此作用。因此，在超过C端五肽的部分中必须存在2个脯氨酸残基或1个脯氨酸和1个碱性氨基酸。蛙眼肽（在哺乳动物中偏好NK3）对SCC的刺激作用可被CP99994和SR48968（NK1和NK2拮抗剂）降低，而不受SB222200（NK3拮抗剂）影响。这三种拮抗剂均不影响蛙皮素对SCC的刺激作用。