Peres M, Bourgeois D, Roussel S, Lefur Y, Devoulon P, Remy C, Barrere B, Decorps M, Pinard E, Riche D
Laboratoire de Recherches Cérébrovasculaires, INSERM U 182, CNRS UA 641, Université Paris VII, France.
NMR Biomed. 1992 Jan-Feb;5(1):11-9. doi: 10.1002/nbm.1940050104.
Two-dimensional 1H spectroscopic imaging and magnetic resonance imaging were used to study focal ischemia induced by middle cerebral artery occlusion in rats. A water suppressing spin-echo sequence was used at 4.7 T. Phase encoding during the spin-echo delay (TE = 272 ms) yielded an 8 x 8 array of 35 microL voxels. The injured area of the brain had a higher lactate level and markedly lower N-acetyl aspartate, creatine and choline levels than did the non-ischemic regions. The spectroscopic imaging data clearly showed the localization of the infarct, which agreed well with both magnetic resonance imaging and the histological data obtained post-mortem. This study demonstrates the potential usefulness of combining magnetic resonance imaging and 1H spectroscopic imaging for studying animal models of stroke, and indicates the suitability of the technique for further pharmacological approaches.
采用二维氢谱成像和磁共振成像技术研究大鼠大脑中动脉闭塞诱导的局灶性缺血。在4.7 T磁场下使用水抑制自旋回波序列。在自旋回波延迟期间（TE = 272 ms）进行相位编码，得到一个8×8阵列的35微升体素。与非缺血区域相比，脑损伤区域的乳酸水平较高，而N-乙酰天门冬氨酸、肌酸和胆碱水平明显较低。谱成像数据清楚地显示了梗死灶的定位，这与磁共振成像和死后获得的组织学数据都非常吻合。本研究证明了将磁共振成像和氢谱成像结合用于研究中风动物模型的潜在实用性，并表明该技术适用于进一步的药理学研究。
