van der Toorn A, Dijkhuizen R M, Tulleken C A, Nicolay K
Department of in Vivo NMR, Bijvoet Center for Biomolecular Research, The Netherlands.
Magn Reson Med. 1996 Dec;36(6):914-22. doi: 10.1002/mrm.1910360614.
The apparent diffusion coefficient (ADC) of choline-containing compounds (Cho), creatine and phosphocreatine (Cre), N-acetyl-aspartate (NAA), lactate, and water was measured in normal rat brain, and in the ischemic and contralateral region of rat brain approximately 3 and 24 h after induction of focal cerebral ischemia. After 3 h of ischemia, the ADC of Cre and NAA in the ischemic region had significantly decreased by 29% and 19%, respectively (P < 0.05). Lactate ADC was also obtained in the ischemic region. After 24 h of focal ischemia, no ADC values could be measured for NAA, Cre and Cho in the ischemic region because their concentrations had become too low. The ADCs of lactate and water in the ischemic volume were virtually identical at 3 and 24 h after occlusion. The experiments suggest that the ADC decrease of water after induction of ischemia is partly caused by changes in the diffusion characteristics of the intracellular compartment.
在正常大鼠脑以及局灶性脑缺血诱导后约3小时和24小时的大鼠脑缺血及对侧区域，测量了含胆碱化合物（Cho）、肌酸和磷酸肌酸（Cre）、N-乙酰天门冬氨酸（NAA）、乳酸和水的表观扩散系数（ADC）。缺血3小时后，缺血区域Cre和NAA的ADC分别显著降低了29%和19%（P<0.05）。在缺血区域也获得了乳酸ADC。局灶性缺血24小时后，由于缺血区域NAA、Cre和Cho的浓度变得过低，无法测量其ADC值。在闭塞后3小时和24小时，缺血区域乳酸和水的ADC实际上是相同的。这些实验表明，缺血诱导后水的ADC降低部分是由细胞内区室扩散特性的变化引起的。
