Song Jian, Yoshida Atsushi, Yamamoto Yoshihiko, Katano Harutaka, Hagihara Keisuke, Oka Shinichi, Kimura Satoshi, Yoshizaki Kazuyuki
Department of Medical Science I, School of Health and Sport Sciences, Osaka University, Osaka, Japan.
Leuk Lymphoma. 2004 Nov;45(11):2343-7. doi: 10.1080/10428190412331283242.
We encountered a case of a rapidly progressive HHV-8-associated solid lymphoma with AIDS-associated Kaposi's sarcoma (KS). HHV-8 DNA load in whole blood cells was analyzed quantitatively by real-time PCR using amplification of the HHV-8-encoded ORF26 gene. Ours is the first observation that the rapid increase in the HHV-8 viral load (from 1.9x10(4) copies/microg to 1.6x10(6) copies/microg in 40 days) in conjunction with low CD4+ cell counts was accompanied by an accelerated clinical disease progression. The results indicate that the quantity of circulating HHV-8 is measurable with real-time PCR and can provide clinically useful information.
我们遇到了一例伴有艾滋病相关卡波西肉瘤（KS）的快速进展性HHV-8相关实体淋巴瘤病例。通过使用HHV-8编码的ORF26基因扩增的实时PCR对全血细胞中的HHV-8 DNA载量进行了定量分析。我们首次观察到，HHV-8病毒载量的快速增加（40天内从1.9×10⁴拷贝/微克增至1.6×10⁶拷贝/微克），同时伴有低CD4⁺细胞计数，这与临床疾病进展加速相关。结果表明，循环中的HHV-8数量可用实时PCR测量，且能提供临床有用信息。
