在未患卡波西肉瘤的人类免疫缺陷病毒感染男性的精液中未检测到人类疱疹病毒8 。
Absence of detectable human herpesvirus 8 in the semen of human immunodeficiency virus-infected men without Kaposi's sarcoma.
作者信息
Diamond C, Huang M L, Kedes D H, Speck C, Rankin G W, Ganem D, Coombs R W, Rose T M, Krieger J N, Corey L
机构信息
Department of Medicine, University of Washington, Seattle, USA.
出版信息
J Infect Dis. 1997 Sep;176(3):775-7. doi: 10.1086/517299.
The prevalence of human herpesvirus 8 (HHV-8)/Kaposi's sarcoma (KS)-associated herpesvirus was investigated in the semen of 99 human immunodeficiency virus (HIV)-infected men (median CD4 cell count, 357/mm3) by use of a polymerase chain reaction (PCR) assay capable of detecting <10 copies of HHV-8 DNA. Of the subjects, 95 (96%) self-identified as men who have sex with men (MSM), and 3 had a history of clinical KS. Seminal cell specimens were negative for HHV-8 in 98 subjects. None of the 26 without KS (27.1% of 96 tested) who were seropositive for HHV-8 by IFA for latency-associated nuclear antigens had HHV-8 detected in their semen. The only subject with any evidence for seminal HHV-8 DNA was seropositive for HHV-8 and had active KS. HHV-8 was detected in 10 (10.4%) of 96 peripheral blood mononuclear cell specimens. The prevalence of HHV-8 DNA by PCR in semen of HIV-infected MSM without KS is low.
通过使用一种能够检测出少于10份人疱疹病毒8型（HHV-8）/卡波西肉瘤（KS）相关疱疹病毒DNA拷贝的聚合酶链反应（PCR）检测法，对99名感染人类免疫缺陷病毒（HIV）的男性（CD4细胞计数中位数为357/mm³）精液中的HHV-8流行情况进行了调查。在这些受试者中，95名（96%）自认为是男男性行为者（MSM），其中3人有临床KS病史。98名受试者的精细胞标本HHV-8检测呈阴性。通过间接荧光抗体法（IFA）检测潜伏相关核抗原呈HHV-8血清阳性的26名无KS者（占96名检测者的27.1%），其精液中均未检测到HHV-8。唯一有精液HHV-8 DNA证据的受试者HHV-8血清呈阳性且患有活动性KS。在96份外周血单核细胞标本中，有10份（10.4%）检测到HHV-8。在未患KS的HIV感染MSM的精液中，通过PCR检测到HHV-8 DNA的流行率较低。
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