Lemaire Marc, Wésolowski-Louvel Micheline
UMR 5122 Microbiologie et Génétique, Université Claude Bernard Lyon 1, 69622 Villeurbanne, France.
Genetics. 2004 Oct;168(2):723-31. doi: 10.1534/genetics.104.029876.
We isolated a mutant, rag17, which is impaired in glucose induction of expression of the major glucose transporter gene RAG1. The RAG17 gene encodes a protein 87% identical to S. cerevisiae enolases (Eno1 and Eno2). The Kleno null mutant showed no detectable enolase enzymatic activity and has severe growth defects on glucose and gluconeogenic carbon sources, indicating that K. lactis has a single enolase gene. In addition to RAG1, the transcription of several glycolytic genes was also strongly reduced in the DeltaKleno mutant. Moreover, the defect in RAG1 expression was observed in other mutants of the glycolytic pathway (hexokinase and phosphoglycerate kinase). Therefore, it seems that the enolase and a functional glycolytic flux are necessary for induction of expression of the Rag1 glucose permease in K. lactis.
我们分离出了一个突变体rag17，它在主要葡萄糖转运蛋白基因RAG1的葡萄糖诱导表达方面存在缺陷。RAG17基因编码一种与酿酒酵母烯醇化酶（Eno1和Eno2）有87%同源性的蛋白质。Kleno缺失突变体未检测到烯醇化酶的酶活性，并且在葡萄糖和糖异生碳源上存在严重的生长缺陷，这表明乳酸克鲁维酵母有一个单一的烯醇化酶基因。除了RAG1，DeltaKleno突变体中几个糖酵解基因的转录也大幅降低。此外，在糖酵解途径的其他突变体（己糖激酶和磷酸甘油酸激酶）中也观察到了RAG1表达的缺陷。因此，似乎烯醇化酶和功能性糖酵解通量对于乳酸克鲁维酵母中Rag1葡萄糖通透酶的表达诱导是必需的。
